黄芪解毒汤诱导乳腺癌细胞凋亡及对β-catenin、C-myc基因表达影响的研究

Effects of Huangqi Jiedu Decoction on Inducing Apoptosis of Breast Cancer Cells and Gene Expression of β-catenin and C-myc

目的探讨黄芪解毒汤体外干预人乳腺癌细胞MCF-7凋亡及对β-catenin、C-myc基因表达的影响,及该方抑制乳腺癌复发转移的可能机制。方法培养MCF-7细胞并将其分为黄芪解毒汤组、阿霉素组及空白对照组。以黄芪解毒汤浓缩液、阿霉素液及细胞培养液分别干预各组MCF-7细胞24 h后,以流式细胞仪检测其对MCF-7乳腺癌细胞凋亡的影响,实时荧光定量PCR检测其对β-catenin,C-myc基因表达的影响。结果与空白对照组比较,黄芪解毒汤组、阿霉素组可明显诱导MCF-7细胞的凋亡(P<0.01);黄芪解毒汤组及阿霉素组β-catenin、C-myc表达量明显低于空白对照组(P<0.01)。结论黄芪解毒可诱导乳腺癌细胞MCF-7凋亡,显著降低β-catenin、C-myc基因表达,该结果为黄芪解毒汤在乳腺癌术后治疗中的应用提供实验依据。

Objective To observe the effects of Huangqi Jiedu decoction on the inducing apoptosis of MCF-7 and expression of β-catenin, C-myc of human breast cancer cells in vitro and to reveal its mechanism of inhibiting recurrence and metastasis of breast cancer. Methods The cultured MCF-7 cells were assigned into Huangqi Jiedu decoction group, doxorubicin group and control group. After intervention for 24 h by Huangqi Jiedu decoction, doxorubicin and cell cultured filed, the apoptosis of breast cancer cell MCF-7 was determined by flow cytometryt. The expression of β-catenin, C-myc genes was detected by Re-al Time Quantitative-PCR. Results Compared with the control group, Huangqi Jiedu decoction and doxorubicin groups had the more obvious effect on inducing the apoptosis of breast cancer cell MCF-7 （P〈0.01）. The expressions of β-catenin and C-myc protein in Huangqi Jiedu decoction and doxorubicin groups were significantly lower than those in control group （P〈0.01）. Conclusion Huangqi Jiedu decoction could induce the apoptosis of MCF-7breast cancer cell, and significantly prohibit the expression of β-catenin and C-myc genes, which provide the experimental evidence for Huangqijiedu decoction in the treatment for postoperation breast cancer.