电针内关穴预处理对心肌缺血再灌注损伤大鼠线粒体膜电位、腺苷A1受体的影响

Effects of Electroacupuncture at Neiguan Point on Mitochondrion Membrane Potential and Adenosine A1 Receptor in Rats with Myocardial Ischemia-Reperfusion Injury

目的观察电针内关预处理对心肌缺血再灌注损伤(myocardial ischemic reperfusion injury,MIRI)大鼠心肌线粒体膜电位、腺苷A1受体的影响,探讨电针预处理防治MIRI的可能作用机制。方法 40只SD雄性大鼠随机分为假手术、缺血再灌注组(模型组)、电针内关穴组、电针环跳穴组,每组10只。采用冠脉结扎法造模,电针内关组和电针环跳穴组在造模前,给予电针刺激20 min/d,共7 d。采用荧光技术测定心肌细胞线粒体膜电位变化,免疫组化法测腺苷A1受体的表达。结果模型组线粒体膜电位及腺苷A1受体较假手术组明显下降(P<0.01);电针内关穴组线粒体膜电位较模型组、电针环跳穴组及假手术组明显升高(P<0.05);电针内关组腺苷A1受体较模型组、电针环跳穴组和假手术组明显升高(P<0.01);模型组与电针环跳组间无明显差异(P>0.05)。结论电针内关穴预处理可以诱导腺苷A1受体的表达,升高线粒体膜电位,对心肌细胞产生保护作用。

Objective To explore the cardioprotective mechanisms of electroacupuncture （EA） at Neiguan point and ob-serve the effect of mitochondrial membrane potential and adenosine A1 receptors in rats with myocardial ischemia-reperfusion injury. Methods The 40 SD male rats were randomly divided into the sham-operation group, ischemia reperfusion （mod-el group）, EA Neiguan group and EA Huantiao group, 10 rats in each group. The models were established by ligating the coronary artery. The EA Neiguan group and EA Huantiao group were given electroacupuncture stimulation pretreatment 20 min every day lasting for 7 days before establishing MIRI model respectively. The mitochondrial membrane potential was tested by fluorescent technique. The expression of adenosine A1 receptor was determined by Immunohistochemical method. Results The mitochondrial membrane potential and adenosine A1 receptor contents of model group decreased significantly than those of control sham-operation group with obviously statistical difference （P〈0.01）. The mitochondrial membrane potential of EA Neiguan group increased than those of model group, control sham-operation group and EA Huantiao group （P〈0.05）. The adenosine A1 receptor increased significantly than model group, EA Huantiao group and control sham-operation group （P〈0.01）. But there was no significandy difference between model group and EA Huantiao group （P〉0.05）. Conclusion The EA at Neiguan point preconditioning could induce the expression of adenosine A1 receptor, increase mitochondrial membrane poten-tial, and have a protection on myocardial cells.