氯化锂诱变选育3-羟基丙酸高产菌株

Breeding of 3-hydroxypropnic acid high-producing strain by lithium chloride mutagenesis

本文以实验室筛选得到的翰逊德巴利酵母菌(Debaryomyces hansenii)DH01为出发菌株,利用氯化锂诱变选育3-羟基丙酸(3HP)产量高并且可以用于实际生产的突变株。经过两轮氯化锂诱变,筛选得到一株产酸量较高的菌株wt06。在培养温度为28℃,120 r/min的条件下,经过48 h培养后,该菌株3HP的产量最高达到23.70 g/L,是原始菌株产量的5.44倍,且该菌株具有良好的遗传稳定性。

In this study, Debaryomyces hansenii DH01 was used as original strain and LiCl was chosen to generate mutations. The mutant strains of 3-hydroxypropinic acid （3HP） high-producing strain were screened out for the practical production. After treatment with LiCl twice, a mutant strain designated as wt06 was obtained. When fermented at 28 Tl , 120 r/min for 48 h, wt06 could produce 23. 70 g/L of 3 HP, which was 5.44 times as high as that of the original strain DH01. Furthermore, the mutant strain wt06 had good heredity stability.