缓释碳源与净水菌胶囊组合脱氮净水性能研究

Research on co-denitrification performance of slow-release organic carbon source and water purification bacteria-embedded solid capsules

在低碳氮比污水治理中,人工投加碳源和菌剂是必要手段,为此,特开展缓释碳源与净水菌胶囊的组合净水性能研究。试验用缓释碳源为乙酸钠包埋物,采用扫描电子显微镜观察释放前后形貌,并用高效液相色谱法进行检测。净水菌胶囊为包含施氏假单胞菌ADH1、肠杆菌AOZ1与枯草芽孢杆菌BSK9包埋物的Wcapsule和B-capsule。试验水源为河道污水,在水桶中间歇曝气条件下开展试验。以无投加污水为对照,比较直投速溶碳源、直投未包埋菌剂、单独投加缓释碳源、单独投加净水菌胶囊以及组合投加缓释碳源和净水菌胶囊各试验组水体氨氮、总氮、COD等指标变化。结果表明,缓释碳源释放乙酸钠后内部结构变疏松,形成大量刨花样卷曲片层结构。净水试验中:投加缓释碳源组总氮的去除率达到60.2%,且试验过程中COD始终低于20 mg·L^(-1);投加固定化菌剂W-capsule组总氮去除率达39.1%;组合投加缓释碳源和净水菌胶囊组总氮去除率可达85.2%。可见,缓释碳源与净水菌胶囊组合在河道污水脱氮上表现出较高性能,应用潜力巨大。

For the treatment of wastewater with low carbon and high nitrogen, the indispensable and effective manner is to launch carbon and microbial inoculum. In this study, investigations were performed into the water purifying ca-pacity of a novel and comprehensive management strategy, which integrated sustained-release carbon source and wa-ter purification bacteria-embedded solid capsules. Sodium acetate was used as the carbon source and embedded with carrier materials. Scanning electron microscopy （ SEM） was carried out to analyze micro-morphological changes to the entrapments before and after release of the carbon source. The released carbon source （sodium acetate） was detected using high performance liquid chromatography （ HPLC） . The bacteria-embedded solid capsules were divided into W-capsule and B-capsule, which included Pseudomonas stutzeri ADH1, Enterobacter sp. A0Z1 and Bacillus subtilis BSK9. Experiments were performed on river sewage in buckets with intermittent aeration to investigate the change to some routine indexes covering ammonia nitrogen, total nitrogen （TN） and chemical oxygen demand （COD） ,etc. Sewage treated using instant/sustained-release carbon source and bacteria with/without embedding were tested as comparable controls. Untreated sewage was also tested as a negative control （NC）. It was shown that extensive wood shaving-like structures formed inside the entrapments after the release of sodium acetate. TN remove rate of the sewage treated with sustained-release carbon source was 60. 2% , and the COD kept less than 20 mg＊L 1. For the sewage treated with W-capsule and the combination of sustained-release carbon source and bacteria-embedded capsules, the TN remove rate was 39. 1 % and 85. 2% , respectively, which indicated high performance of the novel combinatorial strategy in nitrogen removal of river sewage.