哈路比葡萄柚茎段的组培快速繁殖

Rapid Propagation for Hudson Foster Grapefruit Using Its Stems on Tissue Culture

以成年态葡萄柚的茎段为材料,研究不同激素组合、基本培养基对丛生芽增殖的影响,以及再生苗生根、炼苗与移栽技术。结果表明:(1)WPM+6-BA 1.0mg·L^(-1)+NAA 0.2mg·L^(-1)+蔗糖40g·L^(-1)为初代培养的最佳培养基,腋芽诱导率达78.33%;(2)继代增殖的最适培养基为WPM+6-BA 0.75mg·L^(-1)+NAA0.2mg·L^(-1)+蔗糖40g·L^(-1),增殖系数达4.93,芽长达2.62cm;(3)培养基1/2WPM+NAA 0.1mg·L^(-1)+IBA 1.0mg·L^(-1)+蔗糖40g·L-1+AC 0.1%的生根效果最好,生根率达63.33%,株高3.03cm,根粗壮;(4)生根苗移栽成活率达78%以上。

Stem segments of adult Hudson Foster grapefruit plants were used as explants to study the effect of hormones on the primary culture of axillary shoots,as well as that of hormones and media on the proliferation of clustered shoots.In addition,an attempt was made to refine the techniques on rooting,hardening and transplanting of the plantlets.The results showed that:(1)the optimized medium for primary culture was WPM+1.0mg·L^(-1) 6-BA/L+0.2mg·L^(-1) NAA/L+40g·L^(-1) to achieve up to 78.33% induction rate of axillary shoots;(2)the optimized medium for subculture multiplication was WPM+0.75 mg·L^(-1) 6-BA+0.2 mg·L^(-1) NAA+40g·L-1 sucrose to reach a multiplication coefficient of 4.93 with a shoot length of 2.62cm;(3)the optimized medium for rooting was 1/2WPM+0.1mg·L^(-1)NAA+1.0mg·L^(-1) IBA+40g·L^(-1) sucrose+0.1% AC to obtain strong roots at a rooting rate of 63.33% with a plant height up to 3.03cm;(4)the survival rate of transplanted plantlets after rooting was greater than 78%.