人参益智片对神经细胞的保护和修复机制

Study on the mechanism of the protective and repair of nerve cells by the ginseng and intelligence tablet

目的研究人参益智片(RYT)对神经细胞保护和修复的机理,开发人参益智片为辅助改善记忆的保健食品提供依据。方法采用MTT法测定Aβ25-35、冈田酸、过氧化氢致细胞损伤的IC50,并观察人参益智片对SH-SY5Y细胞的保护修复。结果人参益智片与SH-SY5Y细胞共同孵育24 h情况下,在11.72~187.5μg/m L浓度范围内具有促进增殖作用;细胞存活率与冈田酸浓度相关,冈田酸浓度增大,细胞存活率降低,并通过拟合曲线计算IC50为80 nmol/L;人参益智片在一定浓度范围内对SH-SY5Y有保护作用,浓度超过187.5μg/m L时则诱导细胞凋亡,出现细胞毒样作用。结论人参益智片对SH-SY5Y的正常细胞有保护作用,对由OKA损伤的SHSY5Y细胞有修复作用。

Objeotive To study the mechanism of the protection and repair of nerve cells by ginseng and intelligence tablet and provide basis of development of ginseng and intelligence tablet to help to improve memory. Methods IC50 was determined by MTT method, Aβ 25-35, hydrogen peroxide induced cell damage, and the protective and repair of RYT on SH-SY5Y cells were observed. Results RYT and SH-SY5Y ceils incubated 24 hours under, in the 11.72 -187.5 μg/ml concentration range has promoted proliferation; cell survival rate and okadaic acid concentration, okadaic acid concentration increased, cell survival rate decreased and by curve fitting IC50 for 80nmol/L; RYT in a certain range of concentration on SH-SY5Y cells have protective effect, concentration more than 187.5 μg/ml, inducing cell apoptosis, appear cytotoxic effect. Conclusion RYT have a protective effect on normal cells of SH-SY5Y and have a repair effect on SH-SY5Y which is damaged by OKA.