评价ITS、BenA和CaM序列分析在曲霉菌种鉴定方面的应用

Evaluation of ITS,BenA and CaM in identification of clinical Aspergillus

目的评价内转录间隔区(ITS)、β-微管蛋白基因(BenA)和钙调蛋白基因(CaM)序列分析技术对曲霉的鉴定能力。方法对169株曲霉临床分离株分别进行ITS、BenA和CaM序列测定,并在Genbank数据库中进行比对分析以获得其菌种鉴定信息。结果 169株曲霉经ITS、BenA和CaM序列分析分别有52.7%、66.3%、97.6%菌株鉴定至种水平,47.3%、33.7%、2.4%菌株鉴定至属水平,3个序列对曲霉均不存在无法鉴定情况。结论 ITS、BenA和CaM均可用于曲霉菌种鉴定,其中以CaM的鉴定能力最强。

Objective To evaluate the internal transcribed spacer（ITS）,β-tubulin gene（BenA）and calmodulin gene（CaM）sequence analysis in identification of clinical Aspergillus.Methods All 169 strains of Aspergillus collected from clinical patients were analyzed by ITS,BenA,CaM sequencing to obtain the species information with reference to the Genbank database.Results The tested strains were identified to species level with identification rates of 52.7%（89/169）,66.3%（112/169）,97.6%（165/169）and to genus level of 47.3%（80/169）,33.7%（57/169）,2.4%（4/169）by ITS,BenA and CaM,respectively.None of the three sequence markers failed in identification of Aspergillus.Conclusion ITS,BenA and CaM can be used to identify clinical Aspergillus.Among them,CaMis the most efficient molecular marker for Aspergillus identification.