芒果苷协同氟康唑抗耐药白念珠菌作用研究

Synergistic effect of mangiferin in combination with fluconazole against fluconazole-resistant Candida albicans

目的研究芒果苷与氟康唑合用对唑类耐药白念珠菌协同抗真菌的作用和机制。方法采用棋盘式微量稀释法测试芒果苷协同氟康唑对22株耐药白念珠菌的最小抑菌浓度MIC80;时间-杀菌曲线探究两药联用对4株耐药白念珠菌生长的抑制作用;药物生长抑制实验实验探究不同浓度芒果苷和不同浓度氟康唑协同抗耐药白念珠菌药效;通过实时定量RT-PCR检测两药联用时耐药基因CDR1、CDR2、MDR1表达水平。结果芒果苷联合氟康唑可产生协同抗唑类白念珠菌作用,协同指数(FICI)<0.5;两药合用对白念珠菌生长可产生抑制作用;两药合用降低耐药基因CDR1表达水平。结论芒果苷与氟康唑合用可产生协同抗唑类耐药白念珠菌作用。

Objective To explore the synergistic effect of mangiferin in combination with fluconazole against fluconazole-resistant Candida albicans.Methods The minimal inhibitory concentrations（MIC80）of 22fluconazole-resistant Candida albicans strains by mangiferin in combination with fluconazole were determined by checkerboard microdilution assay.Time-kill curves and cell growth test were used to investigate the synergistic effect on Candida albcians growth process.The expression of CDR1,CDR2,MDR1 were measured by Real time RT-PCR.Results Mangiferin in combination with fluconazole could inhibit the growth of Candida albicans obviously（FICI〈0.5）.Mangiferin and fluconazole-treated cells expressed lower level of CDR1 mRNA than the cells grown in the presence of fluconazole or mangiferin alone.Conclusions Mangiferin possessed a synergistic effect with fluconazole against fluconazole-resistant Candida albcians.