硅酸钙复合磷酸钙镁水门汀对成骨细胞早期粘附性能影响的研究

Influence of calcium silicate compound with calcium magnesium phosphate cement on human osteoblast initial adhesion

目的比较研究成骨细胞在磷酸钙镁水门汀(CMPC)和硅酸钙复合磷酸钙镁水门汀(CMPSiC)表面的早期粘附性能。方法测试比较两种水门汀浸提液的主要离子浓度。采用MTT法和细胞形态参数测量法研究两种水门汀材料对MG-63细胞早期粘附能力的影响。结果浸泡后24 h,CMPC组和CMPSiC组的钙、镁、磷离子浓度均明显升高(P<0.05),CMPSiC组的硅离子浓度显著增高(P<0.05)。MTT结果显示,两种水门汀表面粘附细胞的数量均随时间延长而增多,CMPSiC表面粘附的细胞数量明显高于CMPC表面(P<0.05)。接种6 h后,CMPC表面粘附细胞仍呈球状,CMPSiC表面粘附细胞铺展明显,且CMPSiC表面粘附细胞的细胞面积、周长、费雷德直径等细胞形态参数值均明显大于CMPC表面粘附细胞(P<0.05)。结论CMPSiC比CMPC更利于成骨细胞的早期粘附。

Objective To compare the initial adhesive ability of osteoblast which adheres to calcium magnesium phosphate cement （CMPC） and calcium silicate compound with calcium magnesium phosphate cement （CMPSiC）. Methods The ions concentrations of the extract from CMPC and CMPSiC were detected. The initial attachment effects of MG-63 on CMPC and CMPSiC were performed by MTT and cell morphometric evaluations. Results Concentrations of calcium, magnesium and phosphorus ion of the CMPC and CMP- SiC extract significantly increased after immersion for 24 hours （P〈0.05）. And concentration of silicon ion significantly increased in CMPSiC extract（ P〈0.05 ）. The result of MTT experiment indicated that the number of osteoblasts increased over time, and the number adhering on CMPSiC surface was significantly more than that on CMPC surface （P〈 0.05）. After 6 h inoculation, cells adhering on CMPC surface were shrinked, and those on CMPSiC surface were spreading. Cell morphological parameters such as cell surface area, perimeter and Fred diameter of CMPSiC group were significantly larger than those of CMPC group（P〈0.05）. Conclusion Compared with CMPC, CMPSiC is more conducive to initial adhesion and spread of osteoblast.