肺炎支原体IgM抗体三种检测方法的临床应用比较

Comparison of clinical application of three commercial mycoplasma pneumoniae IgM detection method

目的肺炎支原体(MP)是引起非典型性肺炎的常见病原体,IgM抗体检测是诊断肺炎支原体感染的常用方法。本文对被动凝集法、酶联免疫法、磁微粒化学发光法三种商品化的肺炎支原体IgM抗体检测方法临床应用进行比较。方法用Serodia-Myco Ⅱ(Fujirebio)被动凝集法,Sayvon IgM酶联免疫法,安图生物IgM磁微粒化学发光法,检测102例住院患儿的204份样本,比较三种方法检测结果的一致性和准确性。结果 108份凝集法抗体效价≤1∶80的样本,酶联免疫法和磁微粒化学发光法检测均为阴性;64份凝集法抗体效价≥320的样本,酶联免疫法和磁微粒化学发光法检测均为阳性。酶联免疫法和磁微粒化学发光法的符合率为92.2%,S/CO值与凝集法抗体效价呈显著相关性(R=0.84-0.96)。结论临床以凝集法抗体效价≥1∶320或酶联免疫法和磁微粒化学发光法IgM抗体阳性判断肺支急性感染有较高的准确性。

Objectlve It is well known that M. Pneumoniae causes most cases of atypical community - acquired pneumonia, IgM antibody detection is a common method for the diagnosis of MP infection. Our aim is to evaluate the clinical application of three commercial MP IgM detection kit. Methods Totally 204 samples from 102 cases of hospitalized children were detected by FujirebioSerodia - MycolI particle agglutination method, Sayvon ELISA IgM method, and Autobio Microparticle CLIA IgM method. Results Of the 108 PA titer ≤ 1：80 samples, ELISA and Microparticle CLIA detection are negative; 64 PA titer ≥ 320 samples, ELISA and Microparticle CLIA detection are positive. The coincidence rate of ELISA and Microparticle CLIA method is 92.2%, S/CO is significantly correlated with the antibody titer of the PA method （ R = 0.84 - 0.96 ）. Conclusion PA titer （ ≥ 320 ） of M. Pneumoniaeand positive results of ELISA or Microparticle CLIA IgM is a reliable indicator of acute infection of Mycoplasma pneumoniae.