摘要
通过聚合酶链式反应 (PCR)技术 ,以戊肝病毒cDNA为模板将开读框架 3的基因片段扩增并克隆入载体pUC18中。再对扩增片段进行酶切鉴定及测序 ,结果表明此片段与膜板序列的同源性达到 99%以上。将此片段克隆后通过一系列分子生物学技术装入真核胞内表达载体PPIC3及分泌性载体PPIC9中 ,并对载体进行酶切鉴定证实外源基因插入的正确性。
The open read frame 3(ORF3) of the hepatitis E virus was amplified by PCR with the cDNA template.The PCR products were sequenced after cloning into the pUC18 clone vector.The results shows that the homoligy between the ORF3 segment in pUC18 and PCR template was 99.8% at the nucleic acid level.Finally,The ORF3 segment in pUC18 was inserted into PPIC3 and PPIC9 expression vectors of Pichia pastoris yeast.
出处
《微生物学免疫学进展》
2002年第3期6-10,共5页
Progress In Microbiology and Immunology
基金
甘肃省自然科学基金资助项目 (编号 :VS0 0 1 A2 3 0 65 Y)
