GSTM1基因多态性与白血病关系的研究

Relation of GSTM1 Polymorphism with Leukemia

目的:探讨白血病患者GSTM1基因多态性与白血病疗效及主要生物学特征的关系。方法:采用巢式PCR检测GSTM1基因型,比较不同基因型患者第1疗程的缓解率及发病时的主要生物学特征。结果:GSTM1非缺失基因型患者第1疗程的缓解率及部分缓解率与GSTM1缺失基因型无显著性差异(χ~2=0.290,P=0.590);GSTM1基因型与患者性别、年龄,初诊时白细胞数、血红蛋白水平、血小板数、脾脏肿大无相关性(P>0.05);用Log-rank法对GSTM1缺失基因型的AML与ALL组比较结果显示,AML与ALL组患者的生存率之间无统计学差异(χ~2=2.043,P=0.153);GSTM1未缺失基因型患者初诊时血清乳酸脱氢酶浓度与GSTM1缺失基因型比较有统计学差异(P=0.001)。结论:GSTM1基因型与白血病患者第1疗程的缓解及部分缓解率无相关性,GSTM1基因型与患者血清乳酸脱氢酶浓度有关。

Objective： To investigate the relation of GSTM1 polymorphism in leukemia patients with therapeutic efficacy and the main biological characteristics. Methods： The GSTM] genotypes were detected by nested PCR; the remission rate after 1 course of treatment and main biological characteristics at occurrence of leukemia were compared between AL patients with different GSTM1 genotypes, and their relation was analyzed. Results： The remission rate and partial remission rate after 1 course of treatment in patients with GSTMl-undeleted genotype were no significantly different from those in patients with GSTM1 null genotype （ χ^2= 0.290, P 〉 0.05 ）. The stratification analysis showed that GSTM1 null genotype was not related with age, sex, WBC count, Hb level, pit count at initial diagnosis and spleen enlargenent or no（ P 〉 0.05 ）. The comparison of AML and ALL with GSTM1 null genotype by Log-rank showed that the survival rate was no statistically different between AML and ALL patients （χ^2 = 2.043, P 〉 0.05 ）, while the LDH level in serum of patients with GSTMl-undeleted genotype at initial diagnosis was statistically different from that in patients with GSTM1 null genotype （P = 0. 001 ）. Conclusion： The GSTM1 genotype does not relate with remission and partial remission rates after 1 course treatment of AL patients, but relates with LDH level. GSTM1 null genotype deletion may play a role in risk of leukemia.