死亡相关蛋白激酶1在慢性淋巴细胞白血病中的表达

Expression of Death Associated Protein Kinase 1 in Chronic Lymphocytic Leukemia

目的:研究死亡相关蛋白激酶1(DAPK1)在慢性淋巴细胞白血病中的表达水平。方法:用慢性淋巴细胞白血病MEC1细胞系及慢性淋巴细胞白血病患者血液标本提取的B淋巴细胞以研究DAPK1基因表达水平,应用mRNA定量检测和免疫蛋白印迹方法检测mRNA的转录和蛋白的表达情况。结果:mRNA定量和免疫蛋白印迹方法结果表明,慢性淋巴细胞白血病患者体内的DAPK1基因沉默。因此,无论细胞有或没有进行INF-γ处理,MEC1细胞系中DAPK1和自噬相关基因蛋白表达均无明显差异。结论:慢性淋巴细胞白血病中DAPK1基因沉默性表达,使得自噬行为异常。

Objective： To explore the expression of death-associated protein kinase 1 （ DAPK1 ） in chronic lymphocytic leukemia（CLL）. ,Methods： The DAPK1 expression was studied by means of MEC1 ceils and B lymphocytes from blood samples of the patients with CLL. The quantitative detection of mRNA and Western blot were used to detecte the expression of DAPKI and autophagy-related genes at both mRNA and protein levels. Results ： mRNA quantitative detection and Western blot displayed that the DAPK1 expression in the patients with CLL was silenced. So, the expression of DAPK1 and autophagy related genes in MEC1 cells was not significantly different, no matter the cells were treated with or without INF-γ Conclusion： Scilencing of DAPKl expression in CLL results in abnormality of autophagy behavior, thus leading to the occurence of disease.