过敏性紫癜患儿血清中IL-33和可溶性受体的水平及其临床意义

IL-33 and sST2 levels in Serum of Children with Henoch-Schnlein Purpura and Their Clinical Significance

目的:探讨白介素33(IL-33)和可溶性受体(sST2)在过敏性紫癜(allergic purpura,Henoch-Schnlein purpura,HSP)患儿血清中的水平及其临床意义。方法:采用酶联免疫吸附法(ELISA)检测27例HSP患儿和22名正常对照者血清IL-33和sST2水平;应用实时定量RT-PCR方法检测IL-33和sST2水平的mRNA表达。结果:HSP患儿血清IL-33水平为365.5±160.6 pg/ml,较正常对照组175.9±92.8 pg/ml明显升高(P<0.05);而HSP患儿血清中sST2水平为1788.6±523.8 pg/ml,较正常对照组1083.6±489.6 pg/ml亦有升高,但差异无统计学意义(P>0.05),sST2/IL-33比值在HSP患儿明显降低(P<0.05)。RT-PCR结果显示,HSP患儿IL-33 mRNA水平和sST2 mRNA水平较正常对照组分别升高(5.47±1.97)倍和(3.13±2.01)倍,差异均有统计学意义(P<0.05)。sST2/IL-33 mRNA比值在HSP患儿明显降低(P<0.05)。结论:HSP患儿血清IL-33和可溶性受体水平较正常儿童升高,而sST2/IL-33比值较正常儿童明显降低。

Objective： To investigate the serum IL-33 and sST2 levels in children with Henoch-Schonlein purpura （ HSP）, and explore their clinical significance. Methods： Total 27 HSP patients and 22 healthy controls were enrolled in present study. The expressions of IL-33 and sST2 were measured by enzyme linked immunosorbent assay （ELISA）. Using real-time quantitative polymerase chain reaction （ RT - PCR）, the mRNA expression of IL-33 and sST2 were detected in all subjects. Results ： The level of the IL-33 in the serum of HSP group and control group was 365.5 ±160. 6 pg/ml and 175. 9 ±92. 8 pg/ml（P 〈 0. 05）. The level of the sST2 was increased in the serum of HSP group （ 1788.6 ±523.8 pg/ml） as compared with that in control group （ 1083.6 ±489.6 pg/ml） （P 〉 0. 05 ）, but the ratio of sST2/IL-33 in HSP patients was much lower than that in the controls（ P 〈 0. 05 ）, IL-33 and sST2 mRNA levels were up-regulated in HSP patients by 5.47± 1.97 - fold（ P 〈 0. 05 ） and 3.13 ± 2. 01 - fold（ P 〈 0. 05 ） compared with controls, but sST2/IL- 33 significantly decreased in HSP patients（P 〈 0. 05 ）. Conclusion： The levels of IL-33 and sST2 increase in the serum of HSP patients, but the ratio of sST2/IL-33 is much lower than that in control.