巴马小型猪85%肝切除术后急性肝功能衰竭模型的建立

Establishment of a porcine model of acute liver failure after 85% hepatectomy

目的探索不同比例肝切除体积对巴马小型猪急性肝功能衰竭的影响,为建立合适的小型猪大部分肝切除后急性肝功能衰竭模型提供合适的方法。方法分别行75%、85%及95%肝切除,CT检查残留肝并记录存活情况,术前、术后1、3、5 d和术后1、2、3周定期抽血检测肝功能,获取肝组织HE染色,检查肝病理情况。结果75%、85%及95%肝切除小型猪平均存活时间为(19.0±5.6)d,(17.3±5.5)d,(1.3±1.5)d,不同肝切除比例的巴马小型猪病理学评分分别为(5.67±0.52)、(8.17±0.82)、(8.50±0.71)。随着肝切除比例增加,肝功能衰竭发病率升高。85%肝切除可引起谷丙转氨酶、谷草转氨酶、碱性磷酸酶、乳酸脱氢酶、总胆汁酸水平显著增高。结论85%体积肝切除可造成典型的小型猪急性肝功能衰竭模型。

Objective To establish a porcine model of liver failure after different percent hepatectomy. Methods The porcine models of liver failure 75%,85%,95% hepatectomy were developed and the living conditions and survival time were recorded. The blood samples of pre-surgery,post-hepatectomy d1,d3,d5 and post-hepatectomy 1 week,2weeks,and 3 weeks were collected for hepatic function analysis. Histological examination of liver tissues was performed using HE staining. Liver injury histology was interpreted and scored in the terminal samples. Results The average survival time of pigs with post-hepatectomy liver failure after 75%,85%,95% hepatectomy was 19. 0 ± 5. 6 days,17. 3 ± 5. 5days,1. 3 ± 1. 5 days,respectively. Their pathological scores were 5. 67 ± 0. 52,8. 17 ± 0. 82 and 8. 50 ± 0. 71,respectively. With the increase of percent hepatic resection,the incidence of hepatic failure was increasing. ALT,AST,ALP,LDH and TBA were dramatically increased in the pigs after 85% hepatectomy. Conclusions The pig model of acute liver failure by 85% hepatectomy is successfully established,which can cause typical acute liver failure in Bama miniature pigs.