摘要
生物固氮是自然系统中氮的重要来源,可以作为生物肥料,在生产实际中发挥着重要作用。而褐球固氮菌作为自生固氮微生物,广泛存在于土壤中,可以固定空气中的氮气。本文通过用阿须贝(Ashby)无氮培养基培养褐球固氮菌,观察其形态学,发现呈革兰氏阴性,形状呈球杆状或杆状,大小为2.4μm,常成对或八叠状排列,有荚膜,周生鞭毛,能运动。并通过比浊法测定褐球固氮菌的生长曲线,分别在0~6 h为迟缓期,6~16 h为对数生长期,16~20 h为稳定期,20 h后进入衰亡期。
Biological nitrogen fixation is an important source of nitrogen in the natural system, and can be used as a biological fertilizer, which plays an important role in the production practice. Azotobacter chroococcum, which is a kind of nitrogen fixing bacteria, exists widely in the soil. This paper through the use of a Ashby nitrogen free medium A. chroococcum, observe its morphology, found a gram negative, rod-shaped or rod shape of a ball, the size is 2.4 lain, usually paired or eight stacked arrangement, a capsule, week flagella, motile. The growth curve ofA. chroococcum was determined by turbidimetric method, and the growth period was 0 to 6 h, and the logarithmic growth period was from 6 to 16 h, and the stable period was from 16 to 20 h, and then entered the decline stage after 20 h.
出处
《广东化工》
CAS
2017年第8期22-23,共2页
Guangdong Chemical Industry
基金
西南民族大学中央高校基本科研业务费专项资金项目国家重大项目孵化项目(No.2016NZDFH01)
关键词
生物固氮
褐球固氮菌
生长曲线
Biological nitrogen fixation
Azotobacter chroococcum
growth curve