紫杉醇纳米脂质载体包封率的测定

Determination of Encapsulation Efficiency of Paclitaxel Nanoparticles

建立紫杉醇纳米脂质载体包封率测定的高效液相色谱法。选择色谱条件:Diamonsil-C18柱(200mm×4.6 mm,5μm);柱温30℃;流动相:0.067 mol/L磷酸二氢铵水溶液(三乙胺调p H为7.5)-乙腈(3∶2);检测波长205 nm;体积流量1.0 m L/min;进样量20μL。对该色谱条件下测定紫杉醇的方法学进行考察,建立超滤离心法测定紫杉醇纳米脂质载体的包封率。在此色谱条件下紫杉醇与辅料及溶剂峰均得到良好分离,紫杉醇在50.00~1000μg/m L浓度范围内线性关系良好(R=0.9995,n=7),超滤离心法测得紫杉醇纳米脂质载体的包封率为(88.7±0.2)%。该方法准确可靠、简单快速。可用于紫杉醇纳米脂质载体包封率的测定。

Establish high perlbrmance liquid chromatography （HPLC） method for taxol nanostructured lipid carrier the determination of encapsulation efficiency Chromatographic conditions： Diamonsil - C18 column （200 mm×4.6mm, 5microns）; Column temperature 30℃. Mobile phase： 0.067 mol/L ammonium dihydrogen phosphate solution （triethylamine adjust PH to 7.5） - acetonitrile （3：2）; Detection wavelength of 205 nm; The volumetric flow rate 1.0 mL/min; Sample quantity 20 mu L. The method for determination of taxol in tile chrornatograpbic conditions were investigated, establish centrifugal ultrafiltration method to determination taxol nanostructured lipid carrier encapsulation rate. Taxol and accessories and solvent peaks tinder this chromatographic conditions were well separated, taxol in 50.00-1000μg/mL concentration range a good linear relationship （R=0.9995, n=7）, Using the centrifugal ultrafiltration measured taxol nanostructured lipid carrier encapsulation rate was （88.7 ± 0.2）%. The method is accurate and reliable, simple, quick. The method can be used for determination of taxol nanostructured lipid carrier entrapment efficiency.