金胺O荧光染色LED镜检结核分枝杆菌的假阳性研究

Research on the false positivity of the tuberculosis micobacteria checked with LED microscope following dyeing with auramine O fluorescence

目的探讨金胺O荧光染色后用LED显微镜镜检结核分枝杆菌的假阳性率和原因。方法选取宁乡县疾病预防控制中心2015年金胺O荧光染色LED镜检初涂阳性的痰标本用酸性罗氏培养基简单法进行分离培养。结果全年132例金胺O荧光染色镜检初涂阳性标本经培养证实共有3例假阳性病例,假阳性率为2.3%。AFB阳性(报菌数)13例,AFB阳性(+)16例,AFB阳性(++)35例,AFB阳性(+++)46例,AFB阳性(++++)22例。结论 LED镜检假阳性集中出现在镜检报告菌量较少的标本中,食物残渣等其他抗酸物质和标本交叉污染是产生假阳性的主要原因。

Abstract：Objective To explore the false positivity and cause of the micobacteria checked with LED microscope following dyeing with auramine O fluorescence. Methods The initial positive sample of phlegm checked with LED microscope following dyeing with auramine 0 fluorescence in 2015 were selected by Ningxiang center of disease control and prevention. Results Among 132 initial positive samples of phlegm checked with LED microscope following dyeing with auramine O fluorescence, 3 samples had been authenticated as false posilive through fostering. The rate of false positivity was 2.3%.There are 13 cases of AFB positive（provide numher of bacteria）.16 eases of AFB positive（＋）,35 eases of AFB positive（＋＋）,46 cases of AFB positive（＋＋＋）,22 cases of AFB positive（＋＋＋＋）. Conclusion False posilivity checked through LED microscope collectively appears in/he samples with relatively less bacteria in microscope check report. The major cause of false positivity is due to the other antiacid materials in the food remains and cross pollution of samples.