西洋菜锌铁转运蛋白NoZIP1基因的克隆及表达研究

Cloning and expression analysis of NoZIP1 gene in Nasturtium officinale R. Br.

对西洋菜锌铁转运蛋白基因NoZIP1进行克隆及表达分析,为研究其在西洋菜生长发育过程中的生物学功能奠定基础。利用RACE技术克隆西洋菜NoZIP1基因,用生物信息学方法分析获得的基因序列结构,利用实时荧光定量PCR研究NoZIP1基因在不同组织及不同培养条件下的表达特性。结果表明,西洋菜NoZIP1基因c DNA全长1 239 bp,包含完整的阅读框,编码356个氨基酸。实时荧光定量PCR分析显示,NoZIP1基因的表达在锌或者铁胁迫条件下根、叶中均有诱导上调趋势。

To contribute to the further functional determination of the gene involving growth and development of Nasturtium officinale R. Br.,ZIP1 gene was cloned according to homologous gene conserved region by RACE method. Its sequences and expression characters were examined by bioinformatics and real-time PCR,respectively. The full-length cDNA was 1 239 bp and it encoded a polypeptide of 356 amino acids with an entire ORF. It had high similarity to ZIP proteins of other plants. By real-time PCR approach,it was found that the expression of NoZIP1 was significantly induced both in roots and shoots by zinc-deficiency or iron-deficiency.