人工感染PRV仔猪不同组织器官病毒分布及排毒规律研究

Study on the distribution and elimination of swine pseudorabies virus(PRV) in different tissue of pigs artificially infected with PRV

为了研究伪狂犬病毒在猪体不同组织部位的分布和对外排毒规律,将14头50日龄仔猪随机分为实验组(10头)和对照组(4头)。实验组肌内注射半数致死为10^(6.5) TCID_(50)/mL PRV 2 mL,记录不同时间猪只的临床症状,并采用RT-PCR法对攻毒后不同时期各组织器官及血液、鼻拭子、肛门拭子的病毒载量进行研究。结果显示,PRV感染后12 h,试验猪只表现为体温升高、食欲不振、精神萎靡、咳嗽、打喷嚏等症状,攻毒后第3天逐渐出现神经症状;PCR检测表明PRV在体内分布较广,以淋巴结、肺脏、脑组织中病毒载量最高;攻毒不同时期病毒载量的变化规律为先上升后下降的趋势,以第7天或第14天病毒载量最高;对外排毒由高到低依次为鼻拭子、血液、肛门拭子,排毒时间持续35 d。通过研究PRV的分布规律和对外排毒规律,对于揭示PR的发病机制具有重要意义。

To study the expression and elimination characteristics of PRV in difterent tissues, 14 piglets of 50 days were divided into experience group （lO piglets） and contrast group （4 piglets）. The experience group was injected 2 mL PRV with median lethal close of 106.5 TCIDS0/mL. and the clinical symptoms of different swine at different time were recorded. Also the viral loads of tissues, organs, blood swabs, nasal swabs and anal swabs after infection were studied by RT-PCR methods. The results showed thatthe swine infected PRV ＇after 12 h present some symptoms as high body temperature, inappetence, listlessness, cough and sneezing, and emerged neurological symptoms after inoculating PRV 3 days gradually. The highest viral loads were nasals swab, followed by bloods and anal swabs. The highest vir＇,d loads of different tissues or organs were lymph glands, lungs and brains. The viral loads arose after 7 days, then reduced gradually, continuing to 35 d. This study has a great significance in exploring the pathogenesis of PR through the study of the distribution of PRV and external detoxification law.