海洋鱼皮胶原蛋白复合物凝胶制剂对大鼠口腔溃疡的疗效研究

Research on the efficacy of the composite gel collagen from marine fish skin on stomatocace in rats

目的 研究海洋鱼皮胶原蛋白复合物凝胶制剂对大鼠口腔溃疡的疗效。方法 雄性SD大鼠56只，分为海洋鱼皮胶原蛋白复合物凝胶制剂3个给药剂量组、单一凝胶组、单一胶原组、阳性对照组、模型组，共7组，每组8只。采用35%冰醋酸溶液诱发大鼠实验性口腔溃疡模型，观察及测量给药前后各组大鼠口腔溃疡面积变化，电镜观察各组溃疡组织细胞超微结构变化，HE染色观察各组大鼠口腔溃疡组织病理学变化。结果 给药第3天高、中、低剂量组和模型组溃疡面积分别为（8.87±2.11）mm2、（9.50±0.48）mm2、（9.62±1.29）mm2、（12.31±0.69）mm2、第7天高、中、低剂量组和模型组分别为（6.13±2.00）mm2、（7.31±2.70）mm2、（8.92±2.09）mm2、（10.46±1.33）mm2、第10天高、中、低剂量组和模型组分别为（2.52±1.30）mm2、（3.42±1.80）mm2、（4.65±1.51）mm2、（8.62±1.24）mm2、第12天高、中、低剂量组和模型组分别为（0.44±0.92）mm2、（0.41±0.40）mm2、（0.87±1.20）mm2、（3.12±1.71）mm2，海洋鱼皮胶原蛋白复合物凝胶制剂各剂量组溃疡直径均缩小，与模型组比较差异均有统计学意义（P〈0.05），且具有一定剂量依赖关系。电镜观察可见，海洋鱼皮胶原蛋白复合物凝胶制剂各剂量组口腔溃疡部位组织基本愈合，上皮细胞排列紧密，上皮细胞间偶尔见变性细胞，未见炎性细胞。病理观察可见，海洋鱼皮胶原蛋白复合物凝胶制剂各剂量组口腔溃疡部位组织损伤较轻，坏死程度轻，肉芽组织和纤维化形成明显。结论 海洋鱼皮胶原蛋白复合物凝胶制剂对大鼠口腔溃疡具有一定的治疗作用。

Objective To research the therapeutic efficacy of the composite gel collagen from marine fish skin on stomatocace in rats.Methods Fifty-six male SD rats were randomly divided into 7 groups： i. e. the 3 dosages groups （the high dosage, medium dosage and low dosage groups）, the single gel group, the single collagen group, the positive control group and the model group, each consisting of 8 animals. Thirty-five per cent glacial acetic acid solution was used to develop the rat model of stomatocace, then, changes in stomatocace sizes were observed and detected both before and after medication in all the groups. Changes in the ultrastructure of the ulcer-affected cells in various groups were observed under electronmicroscope and pathological changes in the surface area of ulcer-affected tissues in various groups were further observed by HE staining.Results The composite gel collagen from marine fish skin could reduce ulcer sizes of the affected surface areas in various dosage groups. At day 3 after medication, ulcer sizes in the high dosage, medium dosage and low dosage groups as well as the model group were respectively（8.87±2.11）mm2, （9.50±0.48）mm2, （9.62±1.29）mm2 and （12.31±0.69）mm2. At day 7 after medication, ulcer sizes in the high dosage, medium dosage and low dosage groups as well as the model group were respectively（6.13±2.00）mm2, （7.31±2.70）mm2, （8.92±2.09）mm2 and（10.46±1.33）mm2. At day 10 after medication, ulcer sizes in the high dosage, medium dosage and low dosage groups as well as the model group were respectively（2.52±1.30）mm2, （3.42±1.80）mm2, （4.65±1.51）mm2 and（8.62±1.24）mm2. At day 12 after medication, ulcer sizes in the high dosage, medium dosage and low dosage groups as well as the model group were respectively（0.44±0.92）mm2, （0.41±0.40）mm2, （0.87±1.20）mm2 and（3.12±1.71）mm2. Statistical significance could be noted, when comparisons were made between different dosages groups and the model group （P〈 0.05）, furthermore, there was a certain dosage-dependent relationship between them. Electronmicroscopy revealed that the tissues of ulcer-affected areas in various dosage groups were generally healed, epithelial cells were tightly lined and degenerative cells could only be occasionally seen between epithelial cells, but without presence of inflammatory cells. Pathological observation revealed that lesion of the tissues in the ulcer-affected areas of various dosage groups was relatively lighter and tissue necrosis was also relatively lighter. Furthermore, granulation and fibrosis of the affected tissues were quite obvious.Conclusions Composite gel collagen from marine fish skin could produce certain therapeutic efficacy on stomatocace in rats.