转化生长因子β2对人晶状体上皮细胞产生内质网应激及凋亡的影响分析

Effects of transforming growth factor-β2 on the stress and apoptosis of human lens epithelial cells endoplasmlc reticulum

目的 探讨转化生长因子（TGF）β2对人晶状体上皮细胞产生内质网应激及凋亡的影响因素，为降低临床白内障发病率提供依据。方法 体外培养人晶状体上皮细胞，在培养基内加入不同浓度TGF-β2，分析培养基内细胞增殖情况和内质网应激和凋亡相关标志物的表达情况。结果 TGF-β2浓度分别为0、100、200、500ng/L时，对应OD值分别为（0.519±0.017）、（0.459±0.015）、（0.357±0.022）、（0.340±0.028），SR对应为100%、81.46%、61.79%、58.57%，4组实验孔OD值、SR值差异有统计学意义（P〈0.05）；随TGF-β2浓度的增加，caspase-3、GRP78表达率增加，而Hrd1的表达率下降，4组实验孔3种标志性物质表达率比较差异有统计学意义（P〈0.05）。结论 TGF-β2可抑制细胞存活、引发细胞凋亡，同时会导致人晶状体上皮细胞内质网应激和凋亡反应发生。

Objective To investigate the effects of transforming growth factor-β2 （TGF-β2） on human lens epithelial cells endoplasmic reticulum, so as to provide evidence for the decrease of clinical cataracts incidence.Methods Human lens epithelial cells were cultured in vitro with different concentrations of transforming growth factor β2 in the medium, and then, cell proliferation in the medium and the expression of associated markers of endoplasmic reticulum stress and apoptosis were analyzed statistically.Results When TGF-β2 concentrations were respectively at the rates of 0, 100, 200 and 500ng/L, corresponding OD values were respectively （0.519 ± 0.017）, （0.459 ± 0.015）, （0.357 ± 0.022） and （0.340 ± 0.028）. Statistical significance could be seen in the OD and SR values of the 4 experimental wells, when SR values corresponded 100%, 81.46%, 61.79% and 58.57% （P〈0.05）. With the increasing level of TGF-β2, the expression levels of caspase-3 and GRP 78 were increased, while their expression levels were decreased with the increase of Hrd1. There was statistical significance in the expression rates of 3 biomarkers of 4 experimental wells（P〈0.05）.Conclusions TGF-β2 could inhibit cell survival and induce apoptosis, and at the same time, it could cause occurrence of the stress and apoptosis of human lens epithelial cells endoplasmic reticulum.