WT1在结肠癌中的表达及临床意义

Expression and clinical significance of WT1 in colon cancer

目的检测WT1在结肠癌中的表达并探讨其临床意义。方法应用免疫组化及半定量RT-PCR技术检测WT1在48例结肠癌及14例正常结肠组织中的表达情况,分析其与结肠癌患者的临床病理特征及预后等关系。结果 WT1在结肠癌中的阳性表达率明显高于正常组织,差异有统计学意义(P<0.05);WT1的表达与患者的年龄、性别和分化程度无关(P>0.05);而与肿瘤的TNM分期、淋巴结转移相关(P<0.05);生存分析显示WT1阳性表达组患者的生存期与阴性组差异无统计学意义(P>0.05)。结论 WT1的高表达可能在结肠癌的发生发展中起重要作用,设想以WT1为靶点进行基因治疗,有可能为结肠癌的治疗提供新途径。

Objective To observe the expression of WT1 in colon carcinoma, and to explore its clinical significance.Methods The expression levels of WT1 in 48 cases of colon carcinoma and 14 cases of normal colon tissues were detected by immunohistochemistry and semi quantitative RT-PCR. The correlation between the expression of WT1 and clinical pathological features as well as patients prognosis was analyzed. Results The positive expression rates of WT1 in colon carcinoma were significantly higher than those in normal colon tissues （ P 〈0.0 5 ） . The expression of WT1 was not correlated to patients age and sex,differentiation degree of tumor （ P 〉0. 05） , however,which was related with TNM staging and lymph node metastasis of tumor （ P〈0.05）. The survival analysis showed that there was no significant difference in survival time between patients with WT1 positive expression and patients with WT1 negative expression （ P〉0.05 ） . Conclusion The high expression of WT1 may play an important role in the pathogenesis and development of colon cancer, which may provide an new way for treatment of colon cancer.