益气化瘀解毒法对慢性萎缩性胃炎伴异型增生大鼠DNA甲基化酶3b基因的影响

Effects of Qi-tonifying and blood stasis-removing and detoxification therapy on DNMT3b gene in chronic atrophic gastritis rats with dysplasia

目的观察益气化瘀解毒法对慢性萎缩性胃炎伴异型增生模型大鼠DNA甲基化酶3b(DNMT3b)基因的影响。方法将60只SPF级健康雄性Wistar大鼠随机分为造模组50只、空白组10只。造模成功后将造模组剩余的33只大鼠随机分为模型组、维酶素组、消痞颗粒组,每组11只。模型组予0.9%氯化钠注射液,维酶素组予维酶素悬浊液,消痞颗粒组予消痞颗粒中药制备药液,各组均每日灌胃1次,连续治疗12周后检测各组大鼠DNMT3b蛋白表达量。结果维酶素组、模型组DNMT3b蛋白表达量较空白组均明显升高(P<0.01);消痞颗粒组与空白组比较差异无统计学意义(P>0.05)。维酶素组、消痞颗粒组DNMT3b蛋白表达量较模型组降低(P<0.01);消痞颗粒组低于维酶素组(P<0.01)。结论中医益气化瘀解毒法可以逆转胃黏膜异型增生,其作用机制可能是通过下调DNMT3b而实现的。

Objective To observe the clinical effects of Qi-tonifying and blood stasis-removing and detoxification therapy on DNA methylationase 3b（ DNMT3b） in chronic atrophic gastritis rats with dysplasia. Methods60 SPF healthy male Wistar rats were randomly divided into modeling group（ n = 50） and blank group（ n = 10）. After successful modeling,the remaining rats（ n = 33） in modeling group were randomly divided into model group,vitacoenzyme group,Xiaopi granule group,11 rats in each group. The model group was treated by 0. 9% sodium chloride injection,the vitacoenzyme group was treated by vitacoenzyme suspensions and the Xiaopi granule group was treated by Xiaotuqi granule liquid. The rats in each group were intragastric administration one time per day for 12 weeks,and then the protein expression of DNMT3 b was measured. Results The protein expressions of DNMT3 b in vitacoenzyme and model group were significantly higher than that in blank group（ P〈0. 01）,and there was no significant difference between the Xiaopi granule group and the blank group（ P〉0. 05）. The protein expression of DNMT3 b in vitacoenzyme group and Xiaopi granule group was obvious decreased as compared with that in model group（ P〈0. 01）,which in Xiaopi granule group was lower than that in vitacoenzyme group（ P〈0. 01）. Conclusion Qi-tonifying and blood stasis-removing and detoxification therapy can reverse the dysplasia of gastric mucosa,its mechanism may be achieved by down-regulation of DNMT3 b.