非小细胞肺癌患者DC-CIK过继免疫治疗前后外周血TLR4表达的变化

Changes of TLR4 mRNA expression in peripheral blood of patients with non-small cell lung cancer after DC-CIK immunotherapy

目的观察细胞因子诱导的杀伤细胞(DC-CIK)过继免疫治疗前后非小细胞肺癌(NSCLC)患者外周血单个核细胞(PBMC)Toll样受体4(TLR4)mRNA表达变化,及脂多糖刺激下PBMC分泌细胞因子的变化,探讨TLR4在DC-CIK过继免疫治疗中的作用。方法对50例采用DC-CIK治疗的NSCLC患者,运用RT-PCR检测治疗前后PBMC TLR4mRNA表达变化,ELISA法检测治疗前后NSCLC患者PBMC在脂多糖刺激下细胞因子(IL-6、IL-8及TNF-α)分泌的变化。结果 DC-CIK治疗后NSCLC患者PBMC TLR4mRNA的表达水平较治疗前显著降低(t=6.272,P<0.01);脂多糖刺激下PBMC分泌IL-6(t=17.407,P<0.01)、IL-8(t=15.244,P<0.01)及TNF-α(t=12.428,P<0.01)显著减少。结论 DC-CIK过继免疫治疗可能通过降低PBMC TLR4mRNA的表达,提高机体抗肿瘤免疫功能。

Objective To investigate the changes of TLR4 mRNA expression in peripheral blood mononuclear cell（PBMC） of patients with non-small cell lung cancer（NSCLC） after DC-CIK immunotherapy.Methods The mRNA expression of TLR4 in PBMC was detected by reverse transcription PCR（PT-PCR） in 50 patients with NSCLC before and after DC-CIK therapy.The secretion of cytokines（IL-6,IL-8 and TNF-a） of PBMC stimulated with LPS was detected by ELISA before and after DC-CIK therapy.Results After DC-CIK treatment,the relative expression level of TLR4 mRNA in PBMC of NSCLC patients was significantly decreased（t=6.272,P〈 0.01）,the secretion of IL-6（t=17.407,P〈 0.01）,IL-8（t=15.244,P〈 0.01） and TNF-α（t=12.428,P〈 0.01） by PBMC stimulated with LPS was significantly decreased.Conclusion DC-CIK immunotherapy can down-regulate TLR4 expression in PBMC of NSCL patients to enhance the anti-tumor immunity.