丹参酮Ⅰ通过诱导凋亡抑制HepG2细胞生长

TanshinoneⅠInhibits the Growth of HepG2 Cells by Inducing Apoptosis

目的:研究丹参酮Ⅰ对肝癌细胞HepG2生长的影响。方法:MTT法检测丹参酮Ⅰ对HepG2细胞增殖的影响,Western印迹检测经丹参酮Ⅰ处理的HepG2细胞中凋亡标志物c-Parp和周期蛋白D1的表达变化,流式细胞术检测细胞的凋亡及周期分布。结果:MTT分析发现丹参酮Ⅰ可抑制HepG2细胞的生长;经丹参酮Ⅰ处理的HepG2细胞中凋亡标志物c-Parp表达升高,周期蛋白D1表达无明显变化;流式细胞术分析结果进一步证明丹参酮Ⅰ可诱导HepG2细胞发生凋亡,对细胞周期分布无明显影响。结论:丹参酮Ⅰ通过诱导细胞凋亡而抑制HepG2细胞的生长,对细胞周期无明显阻滞,为进一步研究丹参酮Ⅰ的抗肿瘤作用分子机制提供了线索。

Objective：To study the influence of TanshinoneⅠon HepG2 cells proliferation,cell cycle and cell apoptosis. Methods：The proliferation of cells was detected by MTT assay. Western blotting was used to detect the c-Parp and cyclin D1. The cell apoptosis and cell cycle were detected by flow cytometer. Results：MTT results showed that tanshinoneⅠinhibits the growth of HepG2 cells. Western blotting proved that tanshinoneⅠcan make the apoptosis marker c-Parp increase in HepG2 cells,but didn＇t change cyclin D1. Flow cytometry results showed that tanshinoneⅠcan induce apoptosis of HepG2 cells and had no effect on the cycle distribution. Conclusion：TanshinoneⅠ inhibits the growth of HepG2 cells by inducing apoptosis,which provided a clue for further study on the molecular mechanism of anti-tumor action of tanshinoneⅠ.