家蚕的过敏原组分鉴定

Identification of the Allergens of Silkworms

目的:分析引起蚕过敏的组分。方法:利用磷酸缓冲液提取蚕茧和蚕蛹的总蛋白,用SDS-PAGE分析蚕蛋白组分,将蛋白胶转印到PVDF膜上,用蚕过敏患者的阳性血清进行IgE结合试验来鉴定蚕茧和蚕蛹的过敏原组分。结果:蚕茧蛋白可辨条带有8条,其相对分子质量分别约为18×10~3、27×10~3、30×10~3、38×10~3、42×10~3、44×10~3、51×10~3和58×10~3;蚕蛹蛋白主带有6条,其相对分子质量分别约为23×10~3、27×10~3、30×10~3、33×10~3、38×10~3和51×10~3;蚕茧和蚕蛹在相对分子质量约30×10~3和51×10~3附近有2条印迹主带。结论:提示家蚕蛋白中存在特定的过敏原。该研究为蚕过敏的诊断和治疗提供了一定的实验基础。

Objective： To identify the allergen composition in silkworms. Methods： The total proteins of silkworm cocoon and silkworm chrysalis were extracted with PBS, and they were analyzed by SDS-PAGE, and Western blotting to find the allergen composition. Results： The Mr of main protein in silkworm cocoon were about 18× 10^3, 27× 10^3, 30× 10^3, 38× 10^3, 42× 10^3, 44× 10^3 and 51× 10^3. and Mr of main protein in silkworm chrysalis were about 23× 10^3, 27× 10^3, 30× 10^3, 33× 10^3, 38× 10^3 and 51× 10^3. The main detected allergic protein in silkworm cocoon and silkworm chrysalis were about 30× 10^3 and 51× 10^3. Conclusion： There were common allergens in silkworm cocoon and silkworm chrysalis. The study can provide experiment basis for desensitization treatment of inhalant allergy and asthma prevention.