转AtNHX1基因烟草钾积累及根系生理特性

Potassium Accumulation and Root Physiological Characteristics of At NHX1-expressing Tobacco

为了探明转At NHX1基因提高烟叶钾含量的生理原因,利用大田和水培试验,以3个转At NHX1基因阳性纯合株系N7、N9和N10,以及未转化烟草K326(对照)为材料,研究了转At NHX1基因烟草钾含量、根系形态和生理特性。结果表明,转At NHX1基因烟草在大田中长势一致,烟叶含钾量在10~70 d叶龄始终显著高于对照;在水培实验中,转At NHX1基因烟草吸收K^+能力强,具有较大的K^+吸收速率,其根系形态、根系活力、阳离子交换量和ATPase活性均显著高于对照。转At NHX1基因烟草具有富钾基因型烟草钾高效吸收和积累的特征,因而钾含量大大提高。

In order to investigate the physilogical reason for potassium content increasing in leaves of At NHX1 tobacco,using positive At NHX1 gene homozygous strain N7,N9,N10 and non-transgenic control K326 as material under field and hydroponic condition,this paper studied the potassium content,root system morphology and physiological characteristics of At NHX1-expressing tobacco lines. The results showed that At NHX1-expressing tobacco lines grew with unanimous vigor in the fields. The potassium content on leaves at 10 ~ 70 d was remarkably higher than that of the control under filed condition. In hydroponic experiment,the At NHX1-expressing tobacco lines exhibited strong potassium absorption capability and larger maximum potassium absorption rate. The root system morphology and vigor,cation exchange capacity and ATPase activity were significantly higher than that of the control. These results indicated that At NHX1-expressing tobacco lines had effective potassium absorption and accumulation characteristics. Therefore,the potassium content in tobacco leaf was greatly increased.