傣百解体外细胞毒活性评价及其化学成分预实验

In vitro Cytotoxicity Activity and Preliminary Test for Chemical Components from “Dai-Bai-Jie”(Marsdeniatenacissima)

目的:研究傣百解不同溶剂提取物和不同萃取部位的体外抗肿瘤细胞增殖活性,探索活性部位的化学成分。方法:分别以水、50%乙醇水溶液、95%乙醇水溶液3种溶剂对傣百解进行回流提取,采用系统溶剂法对傣百解95%乙醇水溶液提取物进行萃取,采用SRB法测定不同浓度提取物和萃取部位对人胃癌细胞SGC7901、人肝癌细胞HepG2、人宫颈癌细胞Hela、人卵巢癌细胞SKOV3和人胰腺癌细胞SW1990 5种肿瘤细胞增殖的影响;采用试管法、纸反应法等,对傣百解活性部位的化学成分进行初步研究。结果:傣百解抗肿瘤活性部位为乙酸乙酯萃取部位,对SGC7901、HepG2、Hela和SKOV3肿瘤细胞增殖的IC_(50)分别为8.5、20、18.2、12.2μg·mL^(-1)。乙酸乙酯部位可能含有甾体皂苷、挥发油和有机酸等化学成分。结论:傣百解95%乙醇水溶液提取物的乙酸乙酯萃取部位具有广谱细胞毒活性,化学成分以甾体皂苷为主。

Objective： To explore the cytotoxicity activity of different solvent extracts and fractions from ＂Dai-Bai-Jie＂ ）, and identify the chemical components of activity fractions. Methods： ＂Dai-Bai-Jie＂ was extracted by water, 50% ethanol-aqueous and 95% ethanol-aqueous by reflux method. 95% ethanol-aqueous extract were extracted by ethyl acetate and n-butyl alcohol successively. The proliferation of SGC7901, HepG2, Hela, SKOV3 and SW1990 were detected by SRB method, and components of active fraction were studied by tube reaction and paper reaction methods. Results： Ethyl acetate extract was the active fraction, and the IC50 were 8. 5, 20, 18. 2 and 12. 2 μg·mL^-1 in SGC7901, HepG2, Hela, SKOV3 tumor cells. Steroidal saponins, volatile oil and organic acid may be existed in ＂Dai-Bai-Jie＂ . Conclusion： ＂Dai- Bai-Jie＂ has broad-spectrum eytotoxicity activity, and the main chemical components are steroidal saponins.