研究植物乳杆菌对肠道细菌易位的抑制作用

Lactobacillus plantarum prevents bacterial translocation induced by intestinal ischemia and reperfusion injury in rats

目的研究植物乳杆菌对肠道细菌易位的抑制作用。方法将SD大鼠随机分为4组,即假手术对照组,乳杆菌L2灌胃组,缺血再灌注(Ischemia/Reperfusion,I/R)组,乳杆菌预处理+缺血再灌注组。观察各组动物肠黏膜形态病理学变化、肠道菌群变化、细菌易位、血浆细胞因子的水平。结果与假手术对照组相比,缺血再灌注组大鼠肠黏膜有明显病理损伤改变,肠黏膜出现坏死、脱落。肠道内厌氧菌数量显著下降,肠杆菌数量也有所下降。在肝、脾、肾及肠系膜淋巴结中有细菌存在,其易位率达87.5%(P<0.01)。乳杆菌灌胃组大鼠各项指标无显著变化,乳杆菌预处理+I/R组其变化程度较之缺血再灌注组显著减弱。结论植物乳杆菌L2能够抑制肠道缺血再灌注损伤引起的细菌易位,减弱其对肠黏膜屏障的损伤。

Objective To investigate the ability of Lactobacillus plantarum L2 to prevent intestinal ischemia and reperfusion induced bacterial translocation. Methods 32 SD rats were randomly divided into four groups： sham, Lactobacillus intragastric admin- istration, ischemia and reperfusion （I/R） model, Lactobacillus pre-treatment and I/R model. The bacterial translocation, changes of gut microflora, levels of proinflammatory cytokines in plasma and intestinal morphology were observed. Results The increase in bacterial translocation was significant after 4 h I/R treatment （ P 〈 0.01 ） as compared with sham-operated group. There were obvious pathological changes of mucosal morphology in I/R rats and the number of anaerobic bacteria such as Bifidobacteria, Lactobacillus and Bacteroides was significantly reduced. The level of TNF-α in plasma was increased after I/R injury. Lactobacillus plantarum treatment alone had no effect on intestinal barrier function. However, pretreatment of rats with Lactobacillus plantarum L2 significantly abrogated I/R induced intestinal mucosal injury and prevented translocation of bacteria to mesenteric lymph nodes or to remote organs. Conclusions Lactobacillus plantarum L2 has the ability to prevent bacterial translocation and attenuate I/R induced intestinal injury.