高危型HPV感染与宫颈上皮病变组织中MEKK3、NF-κB基因表达的关系

Relationship of high-risk HPV infection with MEKK3 and NF-κB expression in cervical intraepithelial neoplasia tissue

目的:研究宫颈上皮病变组织中高危型HPV感染与MEKK3、NF-κB表达的关系。方法:收集2013年5月~2016年3月期间125例宫颈活检标本进行研究,宫颈炎症标本、宫颈上皮内瘤变标本、宫颈癌标本分别纳入炎症组、CIN组、恶性组,采用HPV-DNA分型检测试剂盒测定HPV分型,采用免疫组化试剂盒测定MEKK3、NF-κB的蛋白表达量,采用荧光定量PCR试剂盒测定MEKK3、NF-κB以及下游分子的mRNA表达量。结果:高危型HPV阳性宫颈组织中MEKK3、NF-κB的蛋白阳性表达率均显著高于高危型HPV阴性宫颈组织,高危型HPV阳性宫颈组织中MEKK3、NF-κB、Bcl-2、XIAP、Bmi-1、TGF-β、Vimentin的mRNA表达量均显著高于高危型HPV阴性宫颈组织;MEKK3和NF-κB阳性表达组织中Bcl-2、XIAP、Bmi-1、TGF-β、Vimentin的mRNA表达量均显著高于MEKK3和NF-κB阴性表达组织。结论:高危型HPV感染会通过MEKK3/NF-κB通路来增加宫颈上皮病变组织中增殖基因Bcl-2、XIAP、Bmi-1以及侵袭基因TGF-β、Vimentin的表达量。

Objective： To study the relationship of high-risk HPV infection with MEKK3 and NF-κB expression in cervical intraepithelial neoplasia tissue.Methods： A total of 125 cases of cervical biopsy specimens between May 2013 and March 2016 were collected, the cervical inflammation specimens, cervical intraepithelial neoplasia specimens and cervical cancer specimens were included in inflammation group, CIN group and malignant group respectively, HPV-DNA typing detection kits were used to determine HPV typing, immunohistochemical kits were used to determine MEKK3 and NF-κB protein expression, and fluorescent quantitative PCR kits were used to determine the mRNA expression of MEKK3, NF-κB and downstream molecules.Results： MEKK3 and NF-κB protein expression in high-risk HPV-positive cervical tissue were significantly higher than those in high-risk HPV-negative cervical tissue, and MEKK3, NF-κB, Bcl-2, XIAP, Bmi-1, TGF-β and Vimentin mRNA expression in high-risk type HPV-positive cervical tissue were significantly higher than those in high-risk HPV-negative cervical tissue;Bcl-2, XIAP, Bmi-1, TGF-β and Vimentin mRNA expression in tissue with positive MEKK3 and NF-κB expression were significantly higher than those in tissue with negative MEKK3 and NF-κB expression.Conclusions： High-risk HPV infection will increase the expression of proliferation genes Bcl-2, XIAP and Bmi-1 as well as invasion genes TGF-β and Vimentin in cervical intraepithelial neoplasia tissue through MEKK3/NF-κB pathway.