溃疡性结肠炎患者肠道菌群含量以及细胞因子、TLRs分子表达情况的检测

Detection of intestinal flora levels as well as cytokine and TLRs molecule expression in patients with ulcerative colitis

目的:研究溃疡性结肠炎(UC)患者肠道菌群含量与细胞因子、TLRs分子表达量的相关性。方法:选择2013年5月~2016年8月期间在湖北省中西医结合医院确诊为UC的患者作为UC组,采集粪便标本及病变肠黏膜组织、正常肠黏膜组织;选择同期健康体检者作为对照组,采集粪便标本。测定肠道菌群含量及肠黏膜组织中细胞因子、TLRs分子的表达量。结果:UC组患者肠道中双歧杆菌、乳酸杆菌的含量显著低于对照组,大肠埃希菌、拟杆菌、肠球菌的含量显著高于对照组;病变肠黏膜中TLR2、TLR4、TLR5、TLR9的蛋白表达量均显著高于正常肠黏膜且双歧杆菌、乳酸杆菌的含量呈负相关,与大肠埃希菌、拟杆菌、肠球菌的含量呈正相关;病变肠黏膜中IL-17、IL-23、HMGB1、Fstl1、TNF-α的蛋白表达量均显著高于正常肠黏膜且与病变肠黏膜中TLR2、TLR4、TLR5、TLR9的蛋白表达量呈正相关。结论:溃疡性结肠炎患者肠道菌群紊乱能够通过增加TLR的表达量来促进炎性因子分泌、激活肠黏膜炎症反应。

Objective：To study the correlation of intestinal flora levels with cytokine and TLRs molecule expression in patients with ulcerative colitis（UC）.Methods：Patients diagnosed with UC in our hospital between May 2013 and August 2016 were selected as UC group,and the feces samples as well as diseased intestinal mucosa tissue and normal intestinal mucosa tissue were collected;the healthy subjects during the same period were selected as the control group,and the feces samples were collected.The intestinal flora levels as well as the expression of cytokines and TLRs molecules in intestinal mucosa tissue were detected.Results：Bifidobacterium and lactobacillus levels in intestinal tract of UC group were significantly lower than those of control group while e.coli,bacteroides and enterococcus levels were significantly higher than those of control group;TLR2,TLR4,TLR5 and TLR9protein expression in diseased intestinal mucosa were significantly higher than those in normal intestinal mucosa,negatively correlated with the bifidobacterium and lactobacillus levels,and positively correlated with e.coli,bacteroides and enterococcus levels;IL-17,IL-23,HMGB1,Fstl1 and TNF-αprotein expression in diseased intestinal mucosa were significantly higher than those in normal intestinal mucosa and positively correlated with TLR2,TLR4,TLR5 and TLR9protein expression in diseased intestinal mucosa.Conclusions：Intestinal flora disorders in patients with ulcerative colitis can increase the expression of TLR to promote inflammatory cytokine secretion and activate intestinal mucosal inflammation.