原花青素对大鼠体内度洛西汀药动学的影响

Effect of Procyanidins on Pharmacokinetics of Duloxetine in Rats

目的探讨原花青素对抗抑郁药度洛西汀在大鼠体内药动学的影响。方法健康雄性SD大鼠15只,随机分为对照组、单次给药组和多次给药组,各5只。多次给药组连续灌胃100mg/kg原花青素8天,对照组每天灌胃相同体积饮用纯净水,单次给药组仅第8天灌胃100mg/kg原花青素,继而每组大鼠均灌胃给予度洛西汀40mg/kg,并按时间点经大鼠尾静脉采集0.2~0.3m L血样,采用HPLC法检测度洛西汀浓度。结果单次给予原花青素对度洛西汀的药动学参数无显著影响;多次给予原花青素后,仅度洛西汀的达峰时间(T_(max))显著减小[(1.40±0.55)h比(2.82±1.10)h、(3.60±0.89)h,P<0.05],其余参数未有显著变化。结论原花青素对度洛西汀的药动学影响小,其与度洛西汀合用治疗抑郁症有待进一步研究。

Objective To investigate the effect of procyanidins on the pharmacokinetics of duloxetine,an antidepressant,in rats.Methods Fifteen healthy male rats were randomly divided into three groups（n=5）：control group,single-dosage group and multiple-dosage group.The multiple-dosage group received 100 mg/（kg·d） procyanidins for 8 days and the single-dosage group received 100mg/kg procyanidins on the 8th day,the control group was given distilled water.After procyanidins,all rats received an oral administration of 40 mg/kg duloxetine.Blood samples were collected via tail vein at the given time points and analyzed for duloxetine concentration by HPLC.Results No significant change was observed except the Tmax of the multiple-dosage group was significantly reduced compared with that of control group and single-dosage group（1.40±0.55 h vs 2.82±1.10 h,3.60±0.89 h,P〈0.05）.Conclusion As a potential antidepressant,procyanidins has modest effect on the pharmacokinetics of duloxetine.Coadministration of these two drugs to treat depression need further investigation.