膝关节表面软骨与游离体表面软骨FT-IR光谱成像的对比研究

The Comparative Research of Knees' Articular Surface Cartilage and Loose Body's Surface Cartilage Using Fourier Transform Infrared Spectroscopic Imaging

目的研究游离体表面软骨与关节软骨的差异,为修复关节软骨缺损提供借鉴。方法以膝关节表面软骨及关节内游离体表面软骨为研究对象,通过FT-IR光谱成像的分析方法及主成分分析法,从分子水平辨别同处于一个关节腔微环境中两种软骨的差别。结果两种软骨中作为填充物的蛋白多糖,没有明显的变化,二阶导数波数位置中1 514cm^(-1)所代表的胶原N-H弯曲振动(实验组为1 516cm^(-1),对照组为1 514cm^(-1))与1 574cm^(-1)所代表的酰胺Ⅱ(实验组为1 575cm^(-1),对照组为1 573cm^(-1))表现出蓝移。主成分散点图显示关节表面软骨主要分布于第一象限,游离体表面软骨主要分布于三四象限。结论游离体表面软骨基质成分尤其是胶原已发生变化,具有一定的缺陷性。

Objective To study the difference between articular surface cartilage and loose body＇s surface cartilage. Methods The knees＇ articular surface cartilage and loose body＇s surface cartilage were collected research objects, u- sing Fourier Transform Infrared Spectroscopic Imaging and Principle Component Analysis to explore the different of the two samples.Results There was no different in the total PGs content of the two samples calculated by an indirec- tion method.N-H bending vibration wavenumber of collagen in the second derivative peaks （in articular surface cartilage is 1 514 cm^- 1 ,in the loose body is 1 516 cm^- 1） and N-H bending vibration and C-N stretching vibration wavenumber of Amide ]1 in the second derivative peaks （in articular cartilage is 1 573 cm-1 ,in the loose body is 1 575 cm ^-1 ） show- ing up an obvious blue shift.PCA shows that the articular surface cartilage was mainly distributed in the first quadrant, and the loose body was mainly distributed in the three or four quadrant.Conclusion The component of loose body sur- face cartilage,especially collagen has some deficient compared with articular surface cartilage.