原子吸收分光光度法测定葡萄糖中铅含量的建立与验证

Establishment and verification of an atomic absorption spectrophotometric method for determination of lead in glucose

目的建立一种定量葡萄糖中铅含量的原子吸收分光光度法，并验证该法的可行性。方法采用标准加入法，建立定量葡萄糖中铅含量的原子吸收分光光度法，并对建立的方法进行线性、准确度／精密度和重复性验证。结果用标准加入法得到的标准曲线线性良好，决定系数〉0．998。铅加样回收率为95％～103％，测定结果的相对标准偏差为4%，表明该法的准确度／精密度和重复性良好。该法测定铅的定量限为1．6×10-7。结论建立了测定葡萄糖中铅含量的原子吸收分光光度法，且该法的线性、精密度、重复性和定量限均符合现行中国药典的要求。

Objective To establish an quantitative detection of lead content in glucose, atomic absorption spectrophotometric method for and validate the feasibility of the method. Methods Standard addition technique was used to establish the atomic absorption spectrophotometric method for determination of lead in glucose. The linearity, accuracy and repeatability of the method were validated. Results The linearity of the method was good, and the linear coefficient of determination was 〉0. 998. The added recoveries of lead were 95%-103%, and the relative standard deviation was 4%, indicating that the accuracy and repeatability of the method were good. Also the limit of quantitation （LOQ） was determined as 1.6 × 10-7. Conclusion The atomic absorption spectrophotometric method for determining lead content in glucose is established and the linearity, accuracy, repeatability and LOQ of the method all meet the requirements of current Chinese pharmacopoeia.