STATs家族在非小细胞肺癌组织中的表达量分析及其潜在靶基因的研究

STATs family expression in non-small cell lung cancer tissue and their potential target genes

目的:研究非小细胞肺癌组织中STATs家族的表达量及STATs潜在的靶基因。方法:选择在我院诊断为非小细胞肺癌的128例患者,收集肺癌组织和癌旁组织,提取蛋白后采用酶联免疫吸附试剂盒测定STATs以及增殖、凋亡、血管新生相关基因的表达量。结果:非小细胞肺癌组织中STAT1的蛋白表达量显著低于癌旁组织,STAT3、STAT5的蛋白表达量显著高于癌旁组织,STAT2、STAT4、STAT6的蛋白表达量与癌旁组织比较无显著性差异;STAT1高表达非小细胞肺癌组织中Bcl-2、BclxL的蛋白表达量显著低于STAT1低表达非小细胞肺癌组织,Caspase-2、Caspase-3、Caspase-7的蛋白表达量显著高于STAT1低表达非小细胞肺癌组织;STAT3、STAT5高表达非小细胞肺癌组织中VEGF-A、VEGFR1、VEGFR2、FGF2、FGFR1的蛋白表达量显著高于STAT3、STAT5低表达非小细胞肺癌组织。结论:非小细胞肺癌组织中STAT1呈低表达、STAT3和STAT5呈高表达,STAT1的靶基因为增殖和凋亡基因,STAT3和STAT5的靶基因为血管新生基因。

Objective： To study the STATs family expression in non-small cell lung cancer tissue and the potential target genes of STATs. Methods： 128 patients diagnosed with non-small cell lung cancer in our hospital between May 2013 and August 2016 were selected, lung cancer tissue and para-carcinoma tissue were collected to extract protein, and then enzyme linked immunosorbent assay kits were used to determine the expression of STATs as well as proliferation, apoptosis and angiogenesis-related genes. Results： STAT1 protein expression in non-small cell lung cancer tissue was significantly lower than that in para-carcinoma tissue, STAT3 and STAT5 protein expression were significantly higher than those in para-carcinoma tissue, and STAT2, STAT4 and STAT6 protein expression were not significantly different from those in para-careinoma tissue; Bcl-2 and Bcl xL protein expression in non-small cell lung cancer tissue with high STAT1 expression were significantly lower thani those in non-small cell lung cancer tissue with low STAT1 expression while Caspase-2, Caspase-3 and Caspase-7 protein expression were significantly higher than those in non-small cell lung cancer tissue with low STAT1 expression; VEGF-A, VEG FR1, VEGFR2, FGF2 and FGFR1 protein expression in non-small cell lung cancer tissue with high STAT3 and STAT5 expression were significantly higher than those in non-small cell lung cancer tissue with low STAT3 and STATS expression. Oonclusion： STAT1 is lowly expressed while STAT3 and STATS are highly expressed in non-small cell lung cancer tissue, the targenes, and the target genes of STAT3 and STATS are angiogenesis genes.