异位子宫内膜组织中基质金属蛋白酶表达、氧化应激反应与盆腔粘连及盆腔痛的相关性

Correlation of matrix metalloproteinase expression and oxidative stress in ectopic endometrial tissue with pelvic adhesion and pelvic pain

目的:研究子宫内膜异位症(EMs)患者异位子宫内膜组织中基质金属蛋白酶表达、氧化应激反应与盆腔粘连及盆腔痛的相关性。方法:选择在我院接受卵巢囊肿剥除术的67例EMs患者并收集异位子宫内膜组织、在位子宫内膜组织,选择同期在我院接受输卵管绝育手术的45例患者并收集正常子宫内膜组织,测定子宫内膜组织中基质金属蛋白酶及其抑制剂的表达量、氧化应激相关分子的含量。结果:异位子宫内膜组织中MMP2、MMP3、MMP7、8-iso-PGF2α、AOPP、8-OHdG的含量显著高于在位子宫内膜组织、正常子宫内膜组织,TIMP1、TIMP2、CAT、PON-1、SOD的含量显著低于在位子宫内膜组织、正常子宫内膜组织;重度疼痛和中度疼痛EMs患者异位子宫内膜组织中MMP2、MMP3、MMP7、8-iso-PGF2α、AOPP、8-OHdG的含量显著高于轻度疼痛EMs患者,TIMP1、TIMP2、CAT、PON-1、SOD的含量显著低于轻度疼痛EMs患者;重度疼痛EMs患者异位子宫内膜组织中MMP2、MMP3、MMP7、8-iso-PGF2α、AOPP、8-OHdG的含量显著高于中度疼痛EMs患者,TIMP1、TIMP2、CAT、PON-1、SOD的含量显著低于中度疼痛EMs患者。结论:异位子宫内膜组织中基质金属蛋白酶的异常高表达以及氧化应激反应的异常激活与盆腔痛的程度密切相关。

Objective,, To study the correlation of matrix metalloproteinase expression and oxidative stress in ectopic endometrial tissue with pelvic adhesion and pelvic pain in patients with endometriosis （EMs）. Methods： 67 patients with EMs who accepted oophorocystectomy in our hospital between May 2014 and April 2016 were selected to collect ectopie endometrial tissue and eutopic endometrial tissue, 45 patients who accepted tubal sterilization in our hospital during the same period were selected to collect normal endometrial tissue, and the expression levels of matrix metalloproteinases and their inhibitors as well as the levels of oxidative stress-related molecules were detected. Results： MMP2, MMP3, MMPT, 8-iso-PGF2 o, AOPP and 8 OHdG levels in ectopic endometrial tissue were significantly higher than those in eutopic endometrial tissue and normal endometrial tissue while TIMP1, TIMP2, CAT, PON-1 and SOD levels were significantly lower than those in eutopic endometrial tissue and normal endometrial tissue; MMP2, MMP3, MMP7, 8-iso-PGF2., AOPP and 8-OHdG levels in ectopic endometrial tissue of EMs patients with severe pain and moderate pain were significantly higher than those of EMs patients with mild pain while TIMP1, TIMP2, CAT, PON-1 and SOD levels were significantly lower than those of EMs patients with mild pain; MMP2, MMP3, MMP7, 8-iso-PGF2α, AOPP and 8-OHdG levels in ectopic endometrial tissue of EMs patients with severe pain were significantly higher than those of EMs patients with moderate pain while TIMP1, TIMP2, CAT, PON-1 and SOD levels were significantly lower than those of EMs patients with moderate pain. Conclusion： The abnormally high expression of matrix metalloproteinases and the abnormal activation of oxidative stress response in ectopic endometrial tissue are closely related to the degree of pelvic pain.