选择性动脉栓塞化疗联合局部放射治疗对晚期肺癌恶性生物学行为的影响

Effect of selective arterial chemoembolization combined with local radiotherapy on the malignant biological behavior of advanced lung cancer

目的:探讨选择性动脉栓塞化疗联合局部放射治疗对晚期肺癌恶性生物学行为的影响。方法:收集本院收治的晚期肺癌患者76例,回顾性分析其治疗方法及相关检查结果,分为接受静脉化疗联合局部放射治疗的对照组、接受选择性动脉栓塞化疗联合局部放射治疗的观察组。治疗后,采用免疫散射比浊法检测两组血清肿瘤标志物含量,采用荧光定量PCR检测肺癌组织中增殖、侵袭、自噬基因mRNA表达量。结果:治疗后,观察组血清中肿瘤标志物CA19-9、CYFRA21-1、GSTP1、CHI3L1的含量显著低于对照组(P<0.05);观察组肺癌组织中增殖基因HOXB7、TEM8的mRNA表达量显著低于对照组,TCF21的mRNA表达量显著高于对照组(P<0.05);观察组肺癌组织中侵袭基因EFEMP1的mRNA表达量显著高于对照组,PAK4、FoxM1、NGAL的mRNA表达量显著低于对照组(P<0.05);观察组肺癌组织中自噬基因LC3、Beclin-1、pULK、PI3KC3、MAPLC3的mRNA表达量显著均高于对照组(P<0.05)。结论:选择性动脉栓塞化疗联合局部放射治疗可降低晚期肺癌恶性程度,抑制肺癌细胞的增殖和侵袭。

Objective： To study the effect of selective arterial chemoembolization combined with local radiotherapy on the malignant biological behavior of advanced lung cancer. Methods： A total of 76 patients with advanced lung cancer treated in our hospital between May 2011 and July 2015 were collected, their treatment and related test results were retrospectively analyzed, and then they were divided into the control group who accepted intravenous chemotherapy combined with local radiotherapy and the observation group who accepted selective arterial chemoembolization combined with local radiotherapy. After treatment, immune scatter turbidimetry was used to detect the serum tumor marker levels of both groups, and fluorescence quantitative PCR was used to detect the proliferation, invasion and autophagy gene mRNA expression in lung cancer tissue. Results： After treatment, serum tumor markers CA19-9, CYFRA21-1, GSTP1 and CHI3L1 levels of observation group were signifi- cantly lower than those of control group （P〈0.05） ; proliferation genes HOXB7 and TEM8 mRNA expression in lung cancer tissue of observation group were significantly lower than those of control group while TCF21 mRNA expression was significantly higher than that of control group （P〈0.05）; invasion gene EFEMP1 mRNA expression in lung cancer of observation group was significantly higher than that of control group while PAK4, FoxM1 and NGAL mRNA expression were significantly lower than those of control group （P〈0.05） ; autophagy genes LC3, Beclin-1, pULK, PI3KC3 and MAPLC3 mRNA expression in lung cancer tissue of observation group were significantly higher than those of control group （P〈0.05）. Qonclusion： Selective arterial ehemoembolization combined with local radiotherapy can reduce the malignant degree of advanced lung cancer and inhibit lung cancer cell proliferation and invasion.