2014年广东省登革热疫情应急控制后期白纹伊蚊抗药性水平调查

Resistance of Aedes albopictus to insecticides in the late stage of emergency control of dengue fever of Guangdong province in 2014

目的调查2014年广东省登革热疫情应急控制后期白纹伊蚊对常用杀虫剂的抗性现状,为科学使用杀虫剂提供依据。方法 2014年10月底至11月初,在登革热疫情控制后期,分别在广州市的海珠区、白云区,佛山市禅城区、南海区,汕头市潮阳区,潮州市潮安区和揭阳市榕城区登革热疫点采集白纹伊蚊幼虫,实验室繁殖1~2代后,采用《蚊虫抗药性检测方法生物测定法》(GB/T 26347-2010)中的敏感基线法之幼虫浸渍法进行检测。结果广州、佛山、汕头、揭阳和潮州等5市(区)白纹伊蚊对双硫磷和残杀威抗性倍数分别为4.92~8.33和2.35~6.23倍,呈低抗水平;对氯菊酯、高效氯氰菊酯和溴氰菊酯的抗性倍数分别为3.53~31.29、8.35~38.69和2.63~37.04倍。广州市对菊酯类杀虫剂大多达到中抗水平,佛山和潮州市大多为低抗水平,揭阳市为低抗水平,汕头市大多为中抗水平。结论白纹伊蚊的防治应坚持综合防制的原则,加强抗药性监测,科学合理使用杀虫剂,避免使用中高抗性药物,预防或延缓抗药性的产生。

Objective To investigate the resistance status of Aedes albopictus to used insecticides in the late stage ofdengue fever emergency control of Guangdong province in 2014, and learn how to prevent the development of resistance.Method sFrom the end of October to the beginning of November 2014, larve of Ae. albopictus were collected in epidemicregions in Haizhu and Baiyun district of Guangzhou city, Chancheng and Nanhai district of Foshan city, Chaoyang districtof Shantou city, Chao′an district of Chaozhou and Rongcheng district of Jieyang city, and were reared about 1 to 2generations in the lab, then were tested along with the susceptible larvae according to the methods of national standard＂Test Method s of mosquito resistance to insecticides-bioassay methods＂（GB/T 26347-2010） to calculate LC50 andresistance index.Results Aedes albopictusof all tested regions had low resistance to temephos and propoxur, the resistantindex was 4.92-8.33 and 2.35-6.23 fold respectively. The resistantce to permethrin, beta cypermethrin, deltamethrinresistance were 3.53-31.29 fold, 8.35-38.69 fold, and 2.63-37.04 fold respectively. Of pyrethroid insecticides, Guangzhoucity have reached the high level resistance mostly, Foshan city, Chaozhou city mostly low level of resistance, Jieyang city forlow level of resistance, Shantou city mostly above the moderate level of resistance against cypermethrin. Aedes albopictus from some cities also have developed resistance to other insecticides in different levels.Conclusion Resistance ofAe. albopictus in the dengue epidemic cities in Guangdong increased very quick to the used insecticides. The control ofAe. albopictus should adhere to the principle of sustainable and integrated management to prevent or delay the developmentof resistance.