摘要
采集闵行地区规模猪场疑似PRRS阳性病料,在Marc-145细胞上进行病毒分离,采用RT-PCR扩增PRRSV高度保守的N基因并测序。结果表明,成功分离到6株PRRSV,为研究闵行地区PRRSV流行株分子变异和致病机理提供了良好的生物素材。
In this study, positive samples of suspicious pigs according to the clinic signs were isolated. Six strains of isolates were identified by the special cytopathic effect of PRRSV on Marc-145 cell line and the similar nucleotide sequence of PRRSV conserved N gene. The isolate PRRSV will be good to us for understanding the molecular changes of the prevailing stains in the field and the the molecular mechanisms of PRRSV pathogenesis.
出处
《湖北农业科学》
2017年第4期704-706,共3页
Hubei Agricultural Sciences
基金
闵行区自然科学研究课题(2015MHZ041)
关键词
猪繁殖与呼吸综合征病毒
N基因
分离与鉴定
序列分析
porcine reproductive and respiratory syndrome virus
isolation and identification
N gene
sequence analysis