聚乙烯醇和海藻酸钠联合固定化汉逊德巴利酵母产3-羟基丙酸的研究

Research on Producing 3-hydroxypropionic Acid by Immobilized Debaryomyces hansenii Using Polyvinyl Alcohol and Sodium Alginate

采用微胶囊固定化技术,利用聚乙烯醇、海藻酸钠和氯化钙制备汉逊德巴利酵母微胶囊,探讨了聚乙烯醇、海藻酸钠、氯化钙的浓度对汉逊德巴利酵母微胶囊的制备工艺及其产3-羟基丙酸量的影响。结果表明,随着聚乙烯醇、海藻酸钠和氯化钙浓度的分别增加,3-羟基丙酸含量先增加后减少。聚乙烯醇最佳浓度为10.0%,此时3-羟基丙酸含量为(20.01±0.66)g/L;海藻酸钠为最佳浓度为1.0%,此时3-羟基丙酸含量为(18.71±0.54)g/L;氯化钙最佳浓度为2.1%,此时3-羟基丙酸含量为(18.37±0.45)g/L。聚乙烯醇和海藻酸钠联合固定汉逊德巴利酵母制备微胶囊最佳工艺为10.0%聚乙烯醇,1.0%海藻酸钠和2.1%氯化钙,在此条件下3-羟基丙酸含量为(21.53±1.12)g/L。

The microencapsulation technology was used to prepare microencapsulated Debaryomyces hansenii by polyving ako- hol,sodium alginate and calcium chloride. The effects of concentration of polyving akohol,sodium alginate and calcium chloride on microcapsules preparation and 3-hydroxypropionic acid production were discussed. The results showed that 3-hydroxy acid content increased firstly and then decreased with increasing concentration of polyving akohol,sodium alginate and calcium chloride respectively. The optimum concentration of polyvinyl alcohol was 10.0% with the content of 3-hydroxy acid, （20.01± 0.66） g/L. The optimum concentration of sodium alginate was 1.0% with the content of 3-hydroxy acid, （18.71±0.54）g/L. The optimum concentration of calcium chloride was 2.1% with the content of 3-hydroxy acid, （18.37±0.45）g/L. The technology of microencapsulated Debaryomyces hansenii by polyving akohol and sodium alginate was polyvinyl alcohol 10.0%,sodium alginate 1.0% and calcium alginate 2.1% with the content of 3-hydroxypropionic acid （21.53±1.12） g/L.