基于金包裹磁性纳米粒子与适配体修饰CdTe纳米探针的凝血酶检测方法

A Novel Method for Detection of Thrombin Using Aptamer-Modified CdTe Nanoparticles Coupled to Au-Magnetic Beads

构建了一个适配体修饰的CdTe纳米探针,利用磁性纳米粒子的分离技术,采用示差脉冲伏安法检测凝血酶。磁性纳米粒子作为分离材料,CdTe纳米粒子作为电化学探针,通过凝血酶的特异性识别,适配体从DNA双链中解旋,并与凝血酶结合形成G-四重体结构,达到检测凝血酶的目的,检出限达0.13pmol/L。该方法灵简便、灵敏、成本低,并成功用于实际样品的检测。此外,该方法可被广泛应用于蛋白质监测和疾病诊断。

We report a new method for detection of thrombin based on aptamer-modified cadmium tellurium nanoprobe using differential pulse voltammetry（DPV）technique.The magnetic nanoparticles were used for separation,and the CdTe nanoparticles acted as electrochemical signal marker.Through the specific recognition for thrombin,aptamer was liberated from the double-strand DNA,and bound with the thrombin to fold into a structure of G-quadruplex.The detection limit reached 0.13pmol/L.This method is demonstrated to be simple,sensitive and low-cost and successfully applied to detection of thrombin in real samples.Furthermore,this method could also be applied to protein monitoring and disease diagnosis.