摘要
采用电化学方法制备了纳米银/聚槲皮素修饰充蜡石墨电极(Ag/Qu/WGE)。以L-苏氨酸(L-Thr)为模板分子,将一定量的壳聚糖,L-Thr和Nafion的混合液涂布在Ag/Qu/WGE上,采用恒电位法电化学清洗除去模板分子L-Thr,得到基于壳聚糖/纳米银/聚槲皮素的L-Thr分子印迹复合膜修饰电极(TMIP/WGE)。采用场发射扫描电镜、红外光谱分析、X射线光电子能谱和电化学技术表征了TMIP/WGE的形成。TMIP/WGE对L-Thr具有良好的电催化氧化作用,可用于L-Thr的快速、灵敏检测,L-Thr的氧化峰电流(1.45 V)和其浓度在0.1~1.0μmol/L范围内呈良好的线性关系,检出限(3σ)为0.01μmol/L。该电极已成功用于苏氨酸发酵液中L-Thr的检测。
A nano-silver/poly quercetin composites modified paraffin-impregnated graphite electrode was prepared by electrochemical method(Ag/Qu/WGE).A mixture of L-Thr,chitosan and Nafion was coated on the surface of Ag/Qu/WGE,L Thr(template molecule) was removed by electrochemical cleaning using potentiostatic method.As a result,a molecular imprinted polymer modified electrode based on chitosan/nano-silver/poly quercetin composites could be obtained for L-Thr detection(TMIP/WGE).The characteristics of the modified electrode were investigated by means of field emission scanning electron microscope technique,X-ray photoelectron spectroscopy,infrared spectra and electrochemical technique.The result indicated that the TMIP/WGE displayed a good electro-catalysis towards the oxidation of L-Thr,which could be used for sensitive and quick detection of L-Thr.The differences of oxidation peak current of L-Thr(1.45 V) showed a good linearity in the concentration range of 0.1-1.0 μmol/L with a detection limit(3σ) of 0.01 μmol/L.The electrode was successfully applied in the determination of L-Thr in fermentation broth.
出处
《分析测试学报》
CAS
CSCD
北大核心
2017年第4期519-523,共5页
Journal of Instrumental Analysis
基金
2014广东省协同创新与平台环境建设专项及公益研究与能力建设专项资金(2014B0901010)