MTA诱导牙髓细胞矿化中Notch信号相关基因的表达

Expression of Notch signaling related gens in human pulp cells stimulated by MTA

目的:通过MTA浸提液作用于人牙髓细胞(h DPCs),探究Notch信号相关基因Notch1、Notch2及Hes1的表达变化。方法:体外培养h DPCs,用0.2 mg/m L MTA浸提液共同培养后,采用Von Kossa染色法及RT-PCR检测其钙化结节的形成情况和不同时间点Notch信号通路相关基因Notch1、Notch2、Hes1、Runx2 mRNA的表达水平。结果:Von Kossa染色结果显示,MTA作用于h DPCs形成钙化结节量明显多于对照组;RT-PCR检测结果显示,Notch1、Notch2 mRNA的表达水平在MTA作用3 d时明显上升(P<0.05);Runx2mRNA表达水平随培养时间延长而升高,7、14 d时明显高于对照组(0 h)(P<0.05);而Hes1 mRNA的表达水平则一直趋于抑制状态,从培养初期就明显低于对照组(0 h)(P<0.05)。结论:Notch信号通路参与了MTA作用下h DPCs矿化调控。

AIM To investigate the effects of mineral trioxide aggregate （ MTA） on the expression ofNotchl, Notch2 and Hesl in human pulp cells （hDPCs）. METHODS： hDPCs were in vitro cultured in the media with 0. 2 mg/mL MTA, the mineralized nodule formation was observed by alizarin red staining. The mRNA expression levels of Notchl,Notch2, Hesl and Runx2 were detected by quantitative RT-PCR. RESULTS： Cells grew normally in the media with 0. 2 mg/mL MTA. MTA treatment produced a Von Kossa positive staining and the formation of miner-alized nodules. The mRNA expression levels of Notchl and Notch2 increased significantly on the 3rd day（P 〈0.05）, then decreased gradually. The expression levels of Runx2 increased gradually over time, and on the 7th and 14th day were significantly higher than that of the control group （0h） （ P〈 0 .0 5 ） ; whereas the expression of Hesl was inhibit-ed compared with the control group （Oh）. CONCLUSION：Notch signaling may be involved in the mineralization regulation of hDPCs^ stimulated by MTA.