摘要
利用高效液相色谱 (HPLC)技术比较了不同生长时期转Bt基因棉花及其对照受体亲本不同器官中主要抗虫黄酮类物质含量的差异 .显著性分析发现 ,外源Bt杀虫蛋白表达对棉花原有的芸香苷、槲皮素和异槲皮苷等抗虫黄酮类化合物不存在不利的影响 .以芸香苷作为黄酮类物质代表物 ,在饲料中进行了棉铃虫幼虫对芸香苷和Bt杀虫蛋白的复合剂量反应试验 .结果表明 ,芸香苷对棉铃虫幼虫的毒杀作用达到极显著水平 ,同时随着芸香苷浓度的提高 ,Bt杀虫毒蛋白对棉铃虫幼虫的杀虫效果得到增强 .芸香苷对Bt杀虫毒蛋白有极显著增效作用 .图 1表 5参
The contents of flavonoids chemicals in Bt transgenic cotton and its parental varieties were analyzed by HPLC method. The results from statistical analysis of variance indicated that Bt toxin in Bt transgenic cotton had no negative effect on synthesis of flavonoids (mainly rutin,isoquercitrin and quercetin ).The results from combined dosage test in vitro showed that rutin had significantly enhanced the resistant effect of Bt-ICP on the cotton bollworm larvae and had very significant resistant effect on the cotton bollworm larvae. For these reasons, it is advantageous to transfer Bt-ICP gene into the cotton with high flavonoids for a better control of the cotton bollworm and preventing evolution of the pests resistance. Fig 1, Tab 5,Ref 12
出处
《应用与环境生物学报》
CAS
CSCD
2002年第4期371-377,共7页
Chinese Journal of Applied and Environmental Biology
基金
国家重点基础研究发展规划项目 (No .G2 0 0 0 0 1 62 0 8)
国家自然科学基金资助项目 (No.39770 4 98)~~
