摘要
芒长是普通小麦的重要农艺性状,受多个基因控制。本研究利用长芒的普通小麦郑麦9023与无芒的西藏半野生小麦Q1028构建一个重组自交系群体(186个株系);采用SSR和DAr T分子标记,构建覆盖小麦全基因组的遗传图谱(2597 cM)。基于重组自交系群体两年芒长表型数据,采用ICIM作图法对小麦芒长性状进行QTL定位分析。共检测到2个与芒长相关的QTL,即Qwa.sau-4AS和Qwa.sau-5AL。它们分别位于4AS和5AL染色体上,可分别解释7.4%和27.3%的表型变异。这2个QTL效应可能分别来源于钩芒基因Hd与抑芒基因B1。利用连锁标记进行基因型分析,表明Qwa.sau-4AS与Qwa.sau-5AL对芒长的抑制效果具有累加效应,且Qwa.sau-5AL效应强于Qwa.sau-4AS。本研究将为精细定位及克隆这2个QTL奠定基础。
Awn length is an important agronomic trait in hexaploid wheat and controlled by multiple genes. A recombinant inbred line(RIL) population containing 186 lines were developed by crossing Tibetan semi-wild wheat accession Q1028(female) and common wheat variety Zhengmai 9023, and a genome-wide genetic map(2597 cM) was constructed using SSR and DAr T markers. QTLs controlling awn length were identified with two-year phenotypic data and the genetic map by using the Inclusive Composite Interval Mapping(ICIM) method. Two QTLs associated with awn length, designated Qwa.sau-4AS and Qwa.sau-5AL, were detected on chromosomes 4A and 5A, which explained 7.4% and 27.3% of phenotypic variations, respectively. According to genetic locations, effects of the two QTLs might be from Hd and B1 genes that could reduce awn length, respectively. Genetic analysis showed that Qwa.sau-4AS and Qwa.sau-5AL had cumulative effects, with stronger inhibiting effect in Qwa.sau-5AL than in Qwa.sau-4AS. These results are valuable for fine mapping and cloning target genes controlling awn length in the future.
出处
《作物学报》
CAS
CSCD
北大核心
2017年第4期496-500,共5页
Acta Agronomica Sinica
基金
国家自然科学基金项目(31570335)资助~~