登革Ⅱ型病毒感染Balb/C实验小鼠的研究

Studies on Den-2 Virus Infection in Balb/C Laboratory Mice

目的 :探讨Balb/C小鼠作为实验动物模型感染登革病毒的情况。方法 :小鼠腹部皮下多点注射接种大剂量登革病毒后在一定时间内检测血清病毒、抗体及腹腔巨噬细胞、脾细胞登革病毒感染情况。结果 :Balb/C实验小鼠接种感染登革病毒以后能产生 1～ 2d病毒血症 ,接种后第 4d血清中即可检测到特异性抗体 ,说明在登革病毒感染早期即产生可检测到的特异性抗体 ,而腹腔巨噬细胞在接种后前 1～ 2d登革病毒感染率较高 ,但下降很快 ,第 4d以后感染水平很低 ,小鼠脾细胞登革病毒感染率极低 ,几乎检测不到阳性。结论

Objective:To understand the mechanisms of Den-2 virus infection on Balb/C laboratory mice. Methods:The laboratory mice were injected intravenously in its abdoman with a defined titers of Den-2 virus. Results:It showed Balb/C laboratory mice could be infected with Den-2 virus, although it was not the susceptible host of dengue virus. It can develop a 1-2 days detectable viremia, Den-2 antibody can be detected in the 4 th day after injected. In the first two days, the infection rates of macrophages in abdomical cavity were high, but dropped rapidly. 4 days later, the rates were very low. Conclusion:Balb/C laboratory mice can be used for study on the animal model infected with Den-2 virus.