摘要
以豌豆幼苗为试材,采用半定量和实时荧光定量RT-PCR技术,研究了不同浓度PEG 6000对肌动蛋白异型体PEAc3表达的影响,为深入了解豌豆幼苗抗旱分子机理以及筛选耐旱豌豆品种奠定基础。结果表明:与正常供水处理相比,15%PEG 6000处理下PEAc3基因的表达量略有升高,20%PEG 6000处理下PEAc3基因的表达量显著升高,低于10%PEG 6000处理,PEAc3的表达量基本没有变化,而25%PEG 6000处理下PEAc3基因的表达量则有所下降。不同干旱条件下PEAc3基因表达量不同这一结果表明,PEAc3基因可能参与豌豆幼苗适应干旱胁迫的生理生化过程,该基因有可能用于豌豆抗旱性强弱的分子筛选。
Pea seedlings were used as experimental materials,and the expression of PEAc3 was studied under different concentrations of PEG 6000by using semi quantitative RT-PCR and real-time fluorescence quantitative RT-PCR technique,which made a good foundation for understanding the molecular mechanism of drought resistance in pea seedlings and select pea cultivars of drought tolerant.The results showed that compared with the normal water supply,the expression of PEAc3 gene was significantly increased under 20%PEG 6000and a slight increase under 15%PEG 6000,and was not changed below 10%PEG 6000.However,the expression of PEAc3gene was decreased under 25%PEG 6000.The expression of PEAc3 gene in different drought conditions showed that the PEAc3 gene might be involved in the physiological and biochemical process of the pea seedling adaptation to drought stress,and the gene might be used for the molecular screening of the drought resistance of pea.
出处
《北方园艺》
CAS
北大核心
2017年第8期107-110,共4页
Northern Horticulture
基金
辽宁省自然科学基金资助项目(2013020072)
