离子色谱法测定正常人血液中的氰化物含量

Determination of Cyanide in Normal Human Blood by Ion Chromatography

在日常案件检验中,经常需要对受害人血液进行氰化物检验,而在正常人血液中也含有微量的氰化物,故正常人血液中氰化物的含量对案件定性具有非常重要的参考价值。取正常人血液加入磷酸,加热使其中的氢氰酸挥发,再用氢氧化钠溶液与氢氰酸反应生成氰化钠,使用离子色谱仪分析溶液中的氰化物,本方法具有分离效果好、灵敏度高的特点。正常人体血液样本264份被分析,检出氰化物204份,检出率76.9%,其中最高值42ng/mL,平均值11ng/mL,有198份样本含有酒精,但含酒精样本的血中氰化物浓度与不舍酒精的血中氰化物浓度比较无明显差异,故可以忽略酒精对血液中氰化物浓度测定的影响。本实验结果可为刑事案件中根据氰化物的含量进行案件定性提供参考。

Cyanide is often tested from the victim＇s blood for casework.However,the normal human blood also contains slight amount of cyanide so that a reference value should be set up on the content of cyanide in normal human blood for the qualitative case.In the experiment reported here,the traditional diffusion approach was adopted in order to avoid transfusing substance and quantities of impurities into detection,the phosphoric acid was added into the normal human blood with a heating treatment followed thereafter to make the hydrocyanic acid volatilized.The hydrocyanic acid was in succession to react with sodium hydroxide to form sodium cyanide,then the cyanide ion was determined by ion chromatography.A total of264 normal human bloods have been analyzed in a series of cases.Of these samples,204 ones showed positive of the cyanide ion,with the average detection rate of 76.9%.The highest concentration value was 42 ng/mL and the mean value 11ng/mL.Although 198 of the samples were containing alcohol,there was no significant difference in the concentration of cyanide ion in blood whether the sample contained alcohol or not.Therefore,the effect of alcohol can be ignored for the determination of cyanide ion in blood.This method is characteristic of good separation and high sensitivity.The results in this article may provide a reference for the relevant cases which demand a qualitative decision about cyanide.