瑶药小钻乙酸乙酯提取物抗类风湿关节炎作用及相关机制探讨

Exploration into the Anti-Rheumatoid Arthritis Mechanism of Ethyl Acetate Extract from Kadsura longipedunculata Finet et Gagnep

目的探讨瑶药小钻乙酸乙酯提取物抗类风湿关节炎(rheumatoid arthritis,RA)作用及相关机制。方法取雄性SD大鼠70只,随机分为正常对照组、模型对照组、雷公藤组、地塞米松组、小钻乙酸乙酯提取物低、中、高剂量组。建立胶原性关节炎(collagen induced arthritis,CIA)大鼠模型,连续灌胃给药28 d,观察大鼠状态、体重、足肿胀度、关节炎指数、踝关节病变;采用ELISA法测定血清白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)及肿瘤坏死因子-α(TNF-α)含量;采用实时荧光定量PCR法检测关节滑膜组织NF-κB p65 mRNA表达。结果小钻乙酸乙酯提取物能显著抑制CIA大鼠足肿胀,降低血清IL-1β、IL-6、TNF-α及滑膜组织NF-κB p65 mRNA水平,作用强于雷公藤,弱于地塞米松,与剂量相关。结论小钻有一定抗RA作用,其机制可能与NF-κB p65转录有关。

OBJECTIVE To research the effect and mechanism of ethyl acetate extract from Kadsura longipedunculata Finet et Gagnep. METHODS The 70 SD male rats were randomly divided into 7 groups： normal control group, model group, tripterginum glycosides （TG） group, dexamethasone （DXM） group, ethyl acetate extract from Kadsura longipedunculata Finet et Gagnep. low, medium and high dosage groups（0. 063,0. 126, 0. 252 g·kg^-1·d^-1 ） , the CIA（ collagen induced arthritis） model of rats was adopted. Under 28 days intragastric administration, the rats＇ state, weight, degree of paw swelling, arthritis index and pathological changes of ankle joints were observed. Their serologic contents of interleukin 115 （ IL-1β ）, interleukin-6 （ IL-6 ）, and tumor necrosis factor-α （TNF-α） were examined by means of ELISA, and the expression of NF-κB p65 mRNA in joint synovial tissues were tested via quantitative Real-time PCR method. RESULTS The ethyl acetate extract from Kadsura longipedunculata Finet et Gagnep. is found obviously effective in inhibiting CIA rats＇ paw swelling, decreasing serologic content of IL-1β, IL-6, and TNF-α, as well as the expression of NF-KB p65 mRNA in synovial tissues. Its effect is dosage-related, and stronger than that of TG, weaker than that of DXM. CONCLUSION Kadsura longipedunculata Finet et Gagnep. is, to some extent, effective against RA, the mechanism of which is related to the transcription of NF-κB p65.